LIX Antibody [Biotin]

• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

LIX Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse LIX
  Val45-Ala118
  Accession # P50228
• Specificity: Detects mouse LIX in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) CXCL6/GCP-2, recombinant rat LIX, recombinant mouse (rm) CXCL13/BLC/BCA-1, rmCXCL11/I-TAC, rmCXCL9/MIG, rmCXCL4/PF4, and rmCXCL12/SDF-1 alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Cxcl5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture: Mouse LIX Antibody (Catalog # MAB433)
  ELISA Detection: Mouse LIX Biotinylated Antibody (Catalog # BAF433)
  Standard: Recombinant Mouse LIX (Catalog # 433-MC)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

CXCL5 (LIX, Liposaccharide-Induced CXC chemokine; also GARG-8) is a secreted 8-9 kDa member of the Intercrine alpha (or CxC) family of chemokines. It is widely expressed, being produced by diverse cell types such as fibroblasts, thymic epithelium, platelets, vascular endothelium, hepatocytes, lung type II alveolar cells and ileal columnar epithelium. As a chemokine, LIX demonstrates chemokinetic properties. It induces the chemotaxis of neutrophils and endothelial cells, and also promotes TNF-alpha secretion from mast cells and macrophages. Notably, circulating LIX is not derived from fibroblasts, but platelets. This suggests that neutrophil homeostasis/chemotaxis is a function of local resident cell activation and LIX secretion, not generally circulating LIX. Mouse LIX is synthesized as a 132 amino acid (aa) precursor that contains a 40 aa signal sequence, a 78 aa mature region (aa 41-118), and a cleavable 14 aa C-terminus. The mature region possesses an ELR/GluLeuArg motif between aa 50-52, and an alpha -family characteristic CxC motif between aa 53-55. Although there are no known splice variants of mouse LIX, considerable proteolytic processing occurs at both the N- and C-termini over aa 41-132. This may reduce the MW in SDS-PAGE by as much as 3 kDa. The majority of LIX appears to start between aa 47-50, and this is positively correlated with bioactivity. Over aa 41-118, mouse LIX shares 73% aa sequence identity with rat LIX. Although not a strict ortholog, mouse LIX shares 63% aa sequence identity with human GCP-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Cxcl5