LIX Antibody

Recombinant Rat CXCL5/LIX (Catalog # 543-RL) chemo­attracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL5/LIX (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CXCL5/LIX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF543). The ND50 is typically 0.3-1.5 µg/mL.

• Reactivity: Rt
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

LIX Antibody Summary

• Immunogen: E. coli-derived recombinant rat LIX
  Ala38-Gln130
  Accession # P97885.1
• Specificity: Detects rat CXCL5/LIX in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse GCP-2 is observed and less than 5% cross‑reactivity with recombinant human GCP-2 is observed and no cross reactivity with _____________ is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Cxcl6
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.3-1.5 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIX Antibody

• LIX

Background

LPS-induced CXC chemokine (LIX) is a chemokine originally cloned from LPS-stimulated mouse fibroblasts. Rat LIX shares approximately 74% amino acid (aa) sequence identity with mouse LIX and is likely the rat orthologue to mouse LIX. Rat LIX cDNA encodes a 130 aa residue precursor with a predicted 37 aa residue signal peptide and a 93 aa residue mature protein. Among human CXC chemokines, rat LIX is most closely related to human GCP-2 and ENA-78. Rat LIX also differs from these two human proteins by having an extended carboxy-terminus. The amino-terminal 115 residues of rat LIX shares 59% and 53% aa sequence homology with human GCP-2 and ENA-78, respectively. It is not clear if LIX should be considered an orthologue of GCP-2 or ENA-78. Yet, mouse LIX was alternatively named mouse GCP-2.

Purified recombinant rat LIX is C-terminally truncated. This is consistent with the observation that natural mouse LIX purified from fibroblasts and epithelial cells also contains multiple amino-terminal and carboxy-terminal truncated isoforms. The shorter isoforms of the natural protein were reported to be more active than the longer forms. The bioactivity of rat LIX can be mediated through CXCR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIX Antibody (AF543)(1)

Publication using AF543

• Miura TA, Wang J, Holmes KV, Mason RJ Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines. Virology, 2007-09-05;369(2):288-98. 2007-09-05 [PMID: 17804032] (ICC, Rat)

Bioinformatics

• Gene Symbol: Cxcl6
• Uniprot:
  • P97885.1()