Livin Antibody (88C570) - BSA Free

Western Blot: Livin Antibody (88C570) [NB100-56548] - detection of Livin in A) Livin transfected cell lysate and B) Molt4 cell lysate. A protein band of apprximate molecular weight of 31 kDa is detected using this antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 88C570
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Livin Antibody (88C570) - BSA Free Summary

• Immunogen: Recombinant human livin protein was used as immunogen.
• Specificity: The Livin antibody, clone 88C570 has been shown to recognize both Livin alpha (~39 kDa) and Livin beta (~37 kDa) isoforms (Nachmias et al, 2003). 2. The antibody also recognizes the 30 kDa (p30-Livin alpha) and 28 kDa (p28-Livin beta) apoptotic cleavage fragments of Livin alpha and beta, respectively (Nachmias et al, 2003). Apoptotic cleavage fragments of Livin have also been referred to as truncated livin (tLivin) (Nachmias et al, 2007). 3. The specificity of the antibody has been validated using Livin shRNA (Quiping et al, 2004).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BIRC7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-5 ug/ml. Use reported in scientific literature (Nachmias et al (2007))
  • Western Blot 1-2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Livin Antibody (88C570) - BSA Free

• baculoviral IAP repeat containing 7
• baculoviral IAP repeat-containing 7
• BIRC7
• KIAP
• KIAPRING finger protein 50
• Kidney inhibitor of apoptosis protein
• livin inhibitor-of-apoptosis
• Livin
• Melanoma inhibitor of apoptosis protein
• ML-IAP
• MLIAPlivin inhibitor of apoptosis
• ML-IAPmliap
• RNF50LIVINbaculoviral IAP repeat-containing protein 7

Background

The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity. Recently, Kasof and Gomes (1), Vucic et al. and Ashhab et al. have identified a novel human inhibitor of apoptosis protein (IAP) family member termed Livin or ML-IAP, which contains a single baculoviral IAP repeat (BIR) domain and a COOH-terminal RING finger domain. Livin gene has two splicing variants that contain open reading frames of 298 and 280 amino acids and both contained a single copy of baculovirus IAP repeat (BIR) and RING domain. Both of the isoforms inhibit TNFa induced apoptosis in Jurkat cells. Livin expression is low in adult tissues. It is relatively expressed at a higher level in developmental tissues and in many cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Livin Antibody (NB100-56548)(22)

Publications using NB100-56548

• Wu S, Xu Q, Sheng W et al. A novel role for Livin in the response to ultraviolet B radiation and pterygium development Int J Mol Med 2020-01-28 [PMID: 32124942] (WB, Human)
• Tchoghandjian A, Souberan A, Tabouret E et al. Inhibitor of apoptosis protein expression in glioblastomas and their in vitro and in vivo targeting by SMAC mimetic GDC-0152 Cell Death Dis 2016-08-05 [PMID: 27490930] (WB, Human)
• Shiloach T, Berens C, Danke C et al. tLivin Displays Flexibility by Promoting Alternative Cell Death Mechanisms. PLoS ONE. 2014-06-25 [PMID: 24960127] (WB, Human)
  
  Details:
  Figs 1B, 2A, 2B, 3B, 3C, 5C, 6C: MelA1, 293T, or A549 cells transfected with truncated Liven (tLiven) constructs. The specificity of the Liven mAb was validated in tLiven transfected cells by WB in Figs 1B, 2A, 2B, 3B, 3C, 5C, 6C.
• Liu X, Wang A, Gao H et al. Expression and role of the inhibitor of apoptosis protein livin in chemotherapy sensitivity of ovarian carcinoma. Int J Oncol. 2012-09-01 [PMID: 22766624]
• Lazar I, Perlman R, Lotem M et al. The clinical effect of the inhibitor of apopotosis protein livin in melanoma. Oncology. 2012-01-01 [PMID: 22441029]
• Slagsvold JE, Pettersen CH, StOrvold GL et al. DHA alters expression of target proteins of cancer therapy in chemotherapy resistant SW620 colon cancer cells. Nutr Cancer. 2010-01-01 [PMID: 20574922] (WB, Human)
  
  Details:
  Livin/BIRC7/kIAP (IMG-347A). WB: Human colon adenocarcinoma (SW620) cell line treated with docosahexaenoic acid (DHA), Fig 2B.
• Lazar I, Yaacov B, Shiloach T et al. The oncolytic activity of Newcastle disease virus NDV-HUJ on chemoresistant primary melanoma cells is dependent on the proapoptotic activity of the inhibitor of apoptosis protein Livin. J Virol. 2010-01-01 [PMID: 19864394] (WB, Human)
  
  Details:
  WB (primary human melanoma cells); Figs 1a, 2, 3, 4a. Note: The specificity of the livin antibody was validated using livin siRNA in Fig 4a.
• Fakler M, Loeder S, Vogler M et al. Small molecule XIAP inhibitors cooperate with TRAIL to induce apoptosis in childhood acute leukemia cells and overcome Bcl-2-mediated resistance. Blood. 2009-02-19 [PMID: 19036706] (WB, Human)
  
  Details:
  WB (human leukemia cell lines), Fig. 1A.
• Wang H, Tan SS, Wang XY et al. Silencing livin gene by siRNA leads to apoptosis induction, cell cycle arrest, and proliferation inhibition in malignant melanoma LiBr cells. Acta Pharmacol Sin. 2007-12-01 [PMID: 18031611] (WB, Human)
  
  Details:
  WB (malignant melanoma human cell line LiBr), Fig 1B (siRNA validated).
• Crnkovic-Mertens I, Wagener N, Semzow J et al. Targeted inhibition of Livin resensitizes renal cancer cells towards apoptosis. Cell Mol Life Sci. 2007-05-01 [PMID: 17437058] (Human)
• Kleinberg L, Lie AK, FlOrenes VA et al. Expression of inhibitor-of-apoptosis protein family members in malignant mesothelioma. Hum Pathol. 2007-07-01 [PMID: 17350081] (WB, Human)
  
  Details:
  WB (frozen effusions from patients with malignant mesothelioma, WM35 human melanoma cells), Fig 1.
• Nachmias B, Lazar I, Elmalech M et al. Subcellular localization determines the delicate balance between the anti- and pro-apoptotic activity of Livin. Apoptosis. 2007-07-01 [PMID: 17294084] (WB, ICC/IF)
  
  Details:
  WB (Figs. 2, 4b, c; 5c, 6a); IF/ICC (Fig 6b, c). Notes: 1. Cells transfected with Livin full-length and truncated constructs were used in this study (all Figures) 2. The antibody is transfected validated by WB for recognition of Livin a, p30-Livin a, Livi
• Gordon G J, Mani M, Mukhopadhyay L et al. Inhibitor of apoptosis proteins are regulated by tumour necrosis factor-alpha in malignant pleural mesothelioma. J Pathol. 2007-03-01 [PMID: 17253597] (Human)
• Gordon G J, Mani M, Mukhopadhyay L et al. Expression patterns of inhibitor of apoptosis proteins in malignant pleural mesothelioma. J Pathol. 2007-03-01 [PMID: 17253596] (Human)
• Choi J, Hwang YK, Sung KW et al. Expression of Livin, an antiapoptotic protein, is an independent favorable prognostic factor in childhood acute lymphoblastic leukemia. Blood. 2007-01-15 [PMID: 16990595] (WB)
  
  Details:
  WB: Whole cell lysate from patients with acute lymphoblastic leukemia (ALL), Fig 2.
• Ma L, Huang Y, Song Z et al. Livin promotes Smac/DIABLO degradation by ubiquitin-proteasome pathway. Cell Death Differ. 2006-12-01 [PMID: 16729033] (WB)
  
  Details:
  1. mAB-Livin (IMG-347A) [WB, Fig 1ABC, Fig 2A, Fig 4B (HeLa cells)].
• Crnkovic-Mertens I, Semzow J, Hoppe-Seyler F, Butz K. Isoform-specific silencing of the Livin gene by RNA interference defines Livin beta as key mediator of apoptosis inhibition in HeLa cells. J Mol Med (Berl). 2006-03-01 [PMID: 16437214]
• Qiuping Z, Jie X, Youxin J et al. Selectively frequent expression of CXCR5 enhances resistance to apoptosis in CD8(+)CD34(+) T cells from patients with T-cell-lineage acute lymphocytic leukemia. Oncogene. 2005-01-20 [PMID: 15580304] (WB)
  
  Details:
  mAB-Livin (IMG-347A) [WB, Fig 6D (T-CLL CD8+ CD34+ T cells, a lymphocytic leukemia cell line)].
• Qiuping Z, Jei X, Youxin J et al. CC chemokine ligand 25 enhances resistance to apoptosis in CD4+ T cells from patients with T-cell lineage acute and chronic lymphocytic leukemia by means of livin activation. Cancer Res. 2004-10-15 [PMID: 15492285] (WB)
  
  Details:
  mAB-Livin (IMG-347A) [WB, Fig 2BC (CD4 and CD8 double-positive thymocytes, T-All and T-CLL CD4+ T cells, and MOLT4 cells.)], [WB, Fig 3C-E].
• Yang QH, Du C. Smac/DIABLO selectively reduces the levels of c-IAP1 and c-IAP2 but not that of XIAP and livin in HeLa cells. J Biol Chem. 2004-04-23 [PMID: 14960576] (WB)
  
  Details:
  1. Livin (IMG- 347A) [WB, Fig 3B (HeLa cells)].
• Crnkovic-Mertens I, Hoppe-Seyler F, Butz K. Induction of apoptosis in tumor cells by siRNA-mediated silencing of the livin/ML-IAP/KIAP gene. Oncogene. 2003-11-13 [PMID: 14614456] (WB)
  
  Details:
  1. Livin (IMG-347A) [WB, Fig.1B (MeWo melanoma, H1299 lung cancer, and HeLa cervical carcinoma cells)].
• Nachmias B, Ashhab Y, Bucholtz V et al. Caspase-mediated cleavage converts Livin from an antiapoptotic to a proapoptotic factor: implications for drug-resistant melanoma. Cancer Res. 2003-10-01 [PMID: 14559822] (WB)
  
  Details:
  mAB-Livin (IMG-347A) [WB, Fig 1A (MeWo cells)], [WB, Fig 2ABCD ( Jurkat T-cell leukemia/lymphoma cells)], [WB, Fig 3CD (breast cancer cells, MCF-7)], [WB, Fig 6AC, 7AC, (melanoma cell line)].

Bioinformatics

• Gene Symbol: BIRC7
• Entrez:
  • 79444(Human)
• Uniprot:
  • Q96CA5()