LIS1 Antibody

Western Blot: LIS1 Antibody [NBP1-03125] - LIS1 in HeLa cell lysate with LIS1 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunocytochemistry/Immunofluorescence: LIS1 Antibody [NBP1-03125] - LIS1 in Jurkat cells with LIS1 antibody at 20 ug/ml.

Immunohistochemistry-Paraffin: LIS1 Antibody [NBP1-03125] - Staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry/Immunofluorescence: LIS1 Antibody [NBP1-03125] - LIS1 in Jurkat cells with LIS1 antibody at 2.5 ug/ml.

Product Discontinued

LIS1 Antibody Summary

• Immunogen: A 14 amino acid synthetic peptide near the C-terminus of human LIS1. The immunogen is located within amino acids 340 - 390 of LIS1.
• Epitope: C-Terminus
• Specificity: an 14 amino acid peptide from near the carboxy terminus of human LIS1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAFAH1B1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Peptide affinity purified

Alternate Names for LIS1 Antibody

• LIS-1
• LIS1PAF-AH alpha
• LIS2
• lissencephaly 1 protein
• Lissencephaly-1 protein
• MDCR
• MDS
• Miller-Dieker syndrome chromosome region
• PAF acetylhydrolase 45 kDa subunit
• PAF-AH 45 kDa subunit
• PAFAH alpha
• PAFAH
• PAFAHA
• platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
• platelet-activating factor acetylhydrolase IB subunit alpha
• platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD)
• platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa
• platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)

Background

PAFAH1B1 was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. PAFAH1B1 encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIS1 Antibody (NBP1-03125)(2)

Publications using NBP1-03125

• Kong HK, Wong MH, Chan HM, Lo SC. Chronic Exposure of Adult Rats to Low Doses of Methylmercury Induced a State of Metabolic Deficit in the Somatosensory Cortex. J Proteome Res. 2013-08-28 [PMID: 23984759] (WB, Rat)
• Lo FY, Chen HT, Cheng HC, Hsu HS, Wang YC. Overexpression of PAFAH1B1 is associated with tumor metastasis and poor survival in non-small cell lung cancer Lung Cancer 2012-06-28 [PMID: 22749159] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: PAFAH1B1
• Entrez:
  • 5048(Human)
  • 18472(Mouse)
  • 83572(Rat)
• Uniprot:
  • P43034(Human)