LIN-28A Antibody

Dot Blot: LIN-28A Antibody [32140002] - The anti-Lin28 antibody (right quadruplicate spots at each panel) or control antibody (left quadruplicate spots at each panel) were printed on membranes at 10 ng/spot. The membranes were incubated with biotinylated cell lysate (50 ug per membrane), followed by probing with streptavidin-HRP and CCD camera image acquisition. (a), Cell lysate from undifferentiated mouse embryonic stem cells; (b), Cell lysate from differentiated mouse embryonic stem cells. Data courtesy of a SDI customer.

Dot Blot: LIN-28A Antibody [32140002] - Protein samples (upper left quadruplicate spots: bovine gamma globulin; upper right quadruplicate spots: cell lysate from 293 cell over expressing Nanog; below left quadruplicate spots: cell lysate from 293 cell over expressing Sox2; below right quadruplicate spots: cell lysate from 293 cell over expressing POU5F1) were printed on membranes at 10 ng/spot. The membrane was incubated with anti-Lin28 antibody (1:1000 dilution) and then probed anti-rabbit IgG-HRP. Signals were acquired with a CCD camera. Data courtesy of a SDI customer.

ELISA: LIN-28A Antibody [32140002]

Product Discontinued

LIN-28A Antibody Summary

• Immunogen: This antibody is specific for the C Terminus Region of the target protein.
• Marker: Undifferentiated human embryonic stem cell Marker
• Specificity: This product is specific for LIN28.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LIN28A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: This antibody is useful in Dot Blot, ELISA and Immunohistochemistry-Paraffin.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for LIN-28A Antibody

• CSDD1
• CSDD1lin-28A
• FLJ12457
• lin-28 homolog (C. elegans)
• lin-28 homolog A (C. elegans)
• LIN28
• LIN-28
• LIN28A
• LIN-28A
• LIN28RNA-binding protein LIN-28
• Tex17
• ZCCHC1
• ZCCHC1protein lin-28 homolog A
• Zinc finger CCHC domain-containing protein 1
• zinc finger, CCHC domain containing 1

Background

LIN28 , also known as CSDD1, ZCCHC1, was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. LIN28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIN-28A Antibody (32140002)(2)

Publications using 32140002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: LIN28A
• Entrez:
  • 79727(Human)
• Uniprot:
  • Q9H9Z2()