LIN-28A Antibody (6D1F9)

Images

 
Western Blot: LIN-28A Antibody (6D1F9) [NBP1-28865] - Analysis using LIN28 mouse mAb against NTERA-2 cell lysate (1).
Immunocytochemistry/ Immunofluorescence: LIN-28A Antibody (6D1F9) [NBP1-28865] - Analysis of methanol fixed Hela cells were transfected with pMX construct of human LIN28, cells were analyzed ~62 hours after transfection.
Immunocytochemistry: LIN-28A Antibody (6D1F9) [NBP1-28865] - Analysis of NTERA-2 cells using LIN28 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, ICC
Clone
6D1F9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

LIN-28A Antibody (6D1F9) Summary

Immunogen
Purified recombinant fragment of LIN28 (aa93-209) expressed in E. Coli (NP_078950).
Marker
Undifferentiated human embryonic stem cell Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LIN28A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunocytochemistry 1:200 - 1:1000
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM Phosphate (pH 7.4)
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Unpurified

Alternate Names for LIN-28A Antibody (6D1F9)

  • CSDD1
  • CSDD1lin-28A
  • FLJ12457
  • lin-28 homolog (C. elegans)
  • lin-28 homolog A (C. elegans)
  • LIN28
  • LIN-28
  • LIN28A
  • LIN-28A
  • LIN28RNA-binding protein LIN-28
  • Tex17
  • ZCCHC1
  • ZCCHC1protein lin-28 homolog A
  • Zinc finger CCHC domain-containing protein 1
  • zinc finger, CCHC domain containing 1

Background

Lin28, also known as CSDD1, ZCCHC1, was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. LIN28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LIN28A
Entrez
Uniprot