LIN-28A Antibody (6D1F9)

Western Blot: LIN-28A Antibody (6D1F9) [NBP1-28865] - Analysis using LIN28 mouse mAb against NTERA-2 cell lysate (1).

Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (6D1F9) [NBP1-28865] - Analysis of methanol fixed Hela cells were transfected with pMX construct of human LIN28, cells were analyzed ~62 hours after transfection.

Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (6D1F9) [NBP1-28865] - Analysis of NTERA-2 cells using LIN28 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Product Discontinued

LIN-28A Antibody (6D1F9) Summary

• Immunogen: Purified recombinant fragment of LIN28 (aa93-209) expressed in E. Coli.
• Marker: Undifferentiated human embryonic stem cell Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LIN28A
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM Phosphate (pH 7.4)
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for LIN-28A Antibody (6D1F9)

• CSDD1
• CSDD1lin-28A
• FLJ12457
• lin-28 homolog (C. elegans)
• lin-28 homolog A (C. elegans)
• LIN28
• LIN-28
• LIN28A
• LIN-28A
• LIN28RNA-binding protein LIN-28
• Tex17
• ZCCHC1
• ZCCHC1protein lin-28 homolog A
• Zinc finger CCHC domain-containing protein 1
• zinc finger, CCHC domain containing 1

Background

Lin28, also known as CSDD1, ZCCHC1, was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. LIN28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIN28A
• Entrez:
  • 79727(Human)
• Uniprot:
  • Q9H9Z2()