LIMPII/SR-B2 Antibody [Alexa Fluor® 647]

• Applications: WB, CyTOF-ready, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

LIMPII/SR-B2 Antibody [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse LIMPII/SR‑B2 Lumenal Loop
  Arg27-Thr432
  Accession # O35114
• Specificity: Detects mouse LIMPII/SR‑B2 Lumenal Loop in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant human (rh) LIMPII is observed and less than 1% cross‑reactivity with rh
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

LIMPII (Lysosomal Integral Membrane Protein II), also known as LPG85 (85 kDa lysosomal membrane sialoglycoprotein) and CD36 antigen-like 2 (CD36L2), is a major lysosomal membrane protein. It belongs to the scavenger receptor class B subfamily and is designated member 2 (SR-B2). Other mammalian members of this family include SR-B1 (alternatively known as Cla-1 and CD36L1), and SR-B3 (CD36) (1‑3). SR-B/CD36 family members are type III integral membrane proteins with an N‑ as well as a C-terminal cytoplasmic tail, and a large extracellular (or luminal in the case of LIMPII) loop containing similarly spaced cysteine residues and multiple glycosylation sites. The C-terminal cytoplasmic tail has a di-leucine-based motif that mediates effective lysosomal targeting. LIMPII is expressed on all tissues and cell types examined so far, including activated platelets. LIMPII binds thrombospondin-1, but the biological significance of this interaction is not known. LIMPII‑thrombospondin interaction may contribute to the pro-adhesive changes of activated platelets during coagulation and inflammation (1). Overexpression of LIMPII causes an enlargement of early and late endosomes, suggesting that LIMPII may play a role in lysosome/endosome biogenesis (4). Mice deficient in LIMPII are impaired in membrane transport processes, resulting in ureteric pelvic junction obstruction, deafness and peripheral neuropathy (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.