Human LIFR alpha ELISA Kit (Colorimetric)

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Standard Curve Reference.
Samples were spiked with high concentrations of Human LIFR alpha, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

Human LIFR alpha ELISA Kit (Colorimetric) Summary

Description
Assay Length: 3 h 30 min
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10
Intra-Assay
CV% < 10
Spike Recovery
80 - 120%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
LIFR

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100x)
  4. Concentrated HRP Conjugate (100x)
  5. Concentrated Wash Buffer (25x)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate (Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Human LIFR alpha ELISA Kit (Colorimetric)

  • CD118 antigen
  • CD118
  • FLJ98106
  • FLJ99923
  • leukemia inhibitory factor receptor alpha
  • leukemia inhibitory factor receptor
  • LIF R alpha
  • LIF R beta
  • LIF receptor
  • LIFR alpha
  • LIFR
  • LIF-R
  • LIFRa
  • SJS2
  • STWS
  • SWS

Background

The LIFR gene encodes a cytokine receptor protien. This protien and a high affinity converter subunit (gp130) form a receptor for mediation of the leukemia inhibitory factor, a factor involved in cellular differentiation and proliferation. If a mutation occurs in this gene, it can cause Schwartz-Jampel syndrom type 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for LIFR alpha ELISA Kit (NBP3-49816). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol LIFR