Lgr6 Antibody Summary
Immunogen |
Synthetic peptide taken within amino acid region 850-920 on human LRG6 protein. |
Localization |
The peptide sequence corresponding to amino acids 860-915 of the human Lgr6 protein |
Specificity |
This antibody detects a fuzzy band of approximately 115 kDa in PC-Lgr6 samples and few additional bands between MW45-60kDa were also observed. The identity of these bands are not known but could be proteolytic fragment of the full-length Lgr6 molecule. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
LGR6 |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA < 1:10000
- Western Blot 1:100-1:2000
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Theoretical MW |
115 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Control Peptide |
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Publications |
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Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%. |
Preservative |
0.02% Sodium Azide |
Purity |
Immunogen affinity purified |
Alternate Names for Lgr6 Antibody
Background
Relaxin family peptides which closely resembles insulin, act on a group of G-protein-coupled receptors now known as relaxin family peptide (RXFP) receptors. The leucine-rich repeat containing RXFP1 and RXFP2 and the small peptide-like RXFP3 and RXFP4 are the physiological targets for relaxin, insulin-like (INSL) peptide 3, relaxin-3 and INSL5, respectively. Leucine rich repeat containing GPCR (Lgr6) is a member of the same large family of GPCR (Lgr1-Lgr16) that binds to variety of glycoprotein hormones like TSH, FSH, LH (1). This family of receptor is unique in having a large ectodomain important for ligand binding. The large extracelluar domain binds the ligands with high affinity; there also exist a low affinity binding site in the exoloop of the trnasmembrane region. LGR6 has a subgroup-specific hinge region after leucine-rich repeats whereas LGR7, like snail LGR, contains a low density lipoprotein (LDL) receptor cysteine-rich motif at the N terminus. The expression of LGR5 is induced in the limb, tail, and intestine by T3 during metamorphosis of frog form larval stage. More importantly, LGR5 mRNA is localized to the developing adult epithelial stem cells of the intestine (2). Lgr6 are also involved in transcription silencing of CpG islands by hypermethylation in colorectal cancer which are referred as methylator phenotypes. The CAN genes include SYNE1, STRAD8, CD109, RNF, RET, APC2 MMP2, PTPRD and Lgr6 (3). Lgr5 is a marker for cycling stem cells in intestine (4) whereas Lgr6 is expressed in earliest embryonic hair placodes (5).Lgr6 positive cells are also involved in wound healing. In adult hair follicles the Lgr6 resides in a region just above the follicle bulge, this region was previously uncharacterized and does not express any known bulge stem cell markers. The Lgr6 is a marker for a distinct population of stem cells that give rise to all lineages of the skin. Unlike Lgr5, the Lgr6 is not regulated by Wnt signaling. The Lgr6-selective antibodies were generated against antigenic peptide taken from human Lgr6 protein corresponding near to carboxy terminal Lgr6 protein. Lgr6-antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for Lgr6 Antibody (NBP1-49695)(1)
Showing Publication 1 -
1 of 1.
Publication using NBP1-49695 |
Applications |
Species |
Barker N, van Es JH, Kuipers J, Kujala P, van den Born M, Cozijnsen M, Haegebarth A, Korving J, Begthel H, Peters PJ, Clevers H. Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature;449(7165):1003-7. 2007-10-25 [PMID: 17934449] |
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Lgr6 Antibody (NBP1-49695). (Showing 1 - 1 of 1 FAQ).
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Can LGR6 be used for skin stem cells detection (CK19)?
- We have not tested this sample specifically, however LGR6 is expressed in skin stem cells. This antibody has been validated in Western blot and ELISA in human, mouse and rat samples. If you are testing your sample using these parameters it should work.
Secondary Antibodies
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Isotype Controls
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