Lgr6 Antibody

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Lgr6 Antibody Summary

• Immunogen: Synthetic peptides corresponding to unique epitope on Lgr6. The peptide sequence corresponding to amino acids 860-915 of the human Lgr6 protein, the peptide was post-synthetically modified to achieve desired antigenicity before coupling to a carrier protein (CAI15929).
• Localization: The peptide sequence corresponding to amino acids 860-915 of the human Lgr6 protein
• Specificity: This antibody detects a fuzzy band of approximately 115 kDa in PC-Lgr6 samples and few additional bands between MW45-60kDa were also observed. The identity of these bands are not known but could be proteolytic fragment of the full-length Lgr6 molecule.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LGR6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • ELISA < 1:10000
• Application Notes: This antibody detects a fuzzy band of approximately 115 kDa in PC-Lgr6 samples and few additional bands between MW45-60kDa were also observed. The identity of these bands are not known but could be proteolytic fragment of the full-length Lgr6 molecule.
• Theoretical MW: 115 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Lgr6 Protein (NBP1-49695PEP)

Reactivity Notes

This product is reactive in Human, Mouse, and Rat.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Lgr6 Antibody

• FLJ14471
• gonadotropin receptor
• GPCR
• leucine-rich repeat containing G protein-coupled receptor 6
• leucine-rich repeat-containing G protein-coupled receptor 6
• leucine-rich repeat-containing G-protein coupled receptor 6
• Lgr6
• VTS20631

Background

Relaxin family peptides which closely resembles insulin, act on a group of G-protein-coupled receptors now known as relaxin family peptide (RXFP) receptors. The leucine-rich repeat containing RXFP1 and RXFP2 and the small peptide-like RXFP3 and RXFP4 are the physiological targets for relaxin, insulin-like (INSL) peptide 3, relaxin-3 and INSL5, respectively. Leucine rich repeat containing GPCR (Lgr6) is a member of the same large family of GPCR (Lgr1-Lgr16) that binds to variety of glycoprotein hormones like TSH, FSH, LH (1). This family of receptor is unique in having a large ectodomain important for ligand binding. The large extracelluar domain binds the ligands with high affinity; there also exist a low affinity binding site in the exoloop of the trnasmembrane region. LGR6 has a subgroup-specific hinge region after leucine-rich repeats whereas LGR7, like snail LGR, contains a low density lipoprotein (LDL) receptor cysteine-rich motif at the N terminus. The expression of LGR5 is induced in the limb, tail, and intestine by T3 during metamorphosis of frog form larval stage. More importantly, LGR5 mRNA is localized to the developing adult epithelial stem cells of the intestine (2). Lgr6 are also involved in transcription silencing of CpG islands by hypermethylation in colorectal cancer which are referred as methylator phenotypes. The CAN genes include SYNE1, STRAD8, CD109, RNF, RET, APC2 MMP2, PTPRD and Lgr6 (3). Lgr5 is a marker for cycling stem cells in intestine (4) whereas Lgr6 is expressed in earliest embryonic hair placodes (5).Lgr6 positive cells are also involved in wound healing. In adult hair follicles the Lgr6 resides in a region just above the follicle bulge, this region was previously uncharacterized and does not express any known bulge stem cell markers. The Lgr6 is a marker for a distinct population of stem cells that give rise to all lineages of the skin. Unlike Lgr5, the Lgr6 is not regulated by Wnt signaling. The Lgr6-selective antibodies were generated against antigenic peptide taken from human Lgr6 protein corresponding near to carboxy terminal Lgr6 protein. Lgr6-antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lgr6 Antibody (NBP1-49695)(1)

Publication using NBP1-49695

• Barker N, van Es JH, Kuipers J, Kujala P, van den Born M, Cozijnsen M, Haegebarth A, Korving J, Begthel H, Peters PJ, Clevers H. Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature;449(7165):1003-7. 2007 Oct 25. [PMID: 17934449]

FAQs for Lgr6 Antibody (NBP1-49695). (Showing 1 - 1 of 1 FAQ).

1. Can LGR6 be used for skin stem cells detection (CK19)?
   • We have not tested this sample specifically, however LGR6 is expressed in skin stem cells. This antibody has been validated in Western blot and ELISA in human, mouse and rat samples. If you are testing your sample using these parameters it should work.

Bioinformatics

• Gene Symbol: LGR6
• Entrez:
  • 59352(Human)
  • 329252(Mouse)
  • 498233(Rat)
• Uniprot:
  • Q9HBX8()