Lgr6 Antibody (JE39-78)

Western Blot: Lgr6 Antibody (JE39-78) [NBP3-32400] - Western blot analysis of Lgr6 on different lysates with Rabbit anti-Lgr6 antibody (NBP3-32400) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 ug/Lane) Lane 2: MCF7 cell lysate (10 ug/Lane) Lane 3: SKOV-3 cell lysate (10 ug/Lane) Lane 4: Mouse testis tissue lysate (20 ug/Lane) Predicted band size: 104 kDa Observed band size: 104 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32400) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Lgr6 Antibody (JE39-78) [NBP3-32400] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-Lgr6 antibody (NBP3-32400) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32400) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Lgr6 Antibody (JE39-78) [NBP3-32400] - Immunocytochemistry analysis of HeLa cells labeling Lgr6 with Rabbit anti-Lgr6 antibody (NBP3-32400) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lgr6 antibody (NBP3-32400) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: JE39-78
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Lgr6 Antibody (JE39-78) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human Lgr6 aa 831-967 / 967. (Uniprot: Q9HBX8)
• Localization: Cell membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LGR6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
• Theoretical MW: 104 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Lgr6 Antibody (JE39-78)

• FLJ14471
• gonadotropin receptor
• GPCR
• leucine-rich repeat containing G protein-coupled receptor 6
• leucine-rich repeat-containing G protein-coupled receptor 6
• leucine-rich repeat-containing G-protein coupled receptor 6
• Lgr6
• VTS20631

Background

LGR6 has been reported to be expressed in rat adrenal, brain, colon, heart, kidney, ovary, oviduct, small intestine, spleen, testis, and uterus Hsu et al., 2000). ESTs have been isolated from human B-cell/lung/testis, blood, brain, colon, eye, germ cell, head/neck, heart, heart/melanocyte/uterus, lung, ovary, stomach, testis, and uterus libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LGR6