Leupaxin Antibody - BSA Free

Western Blot: Leupaxin Antibody [NBP3-06271] - Anti-LPXN rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: PC3 Whole Cell LysateLane C: A431 Whole Cell LysateLane D: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:45 kDa

Immunoprecipitation: Leupaxin Antibody [NBP3-06271] - LPXN was immunoprecipitated using:Lane A: 0.5 mg Jurkat Whole Cell Lysate4 uL anti-LPXN rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-LPXN rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 45 kDaObserved band size :45 kDa

Immunohistochemistry-Paraffin: Leupaxin Antibody - BSA Free [NBP3-06271] - Immunochemical staining of human Leupaxin in human lung with NBP3-06271 at 1:500 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: Leupaxin Antibody - BSA Free [NBP3-06271] - Immunochemical staining of human Leupaxin in human spleen with NBP3-06271 at 1:500 dilution, formalin-fixed paraffin embedded sections.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Leupaxin Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human Leupaxin fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LPXN
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Leupaxin Antibody - BSA Free

• LDLP
• LDPL
• leupaxin

Background

The product encoded by this gene is preferentially expressed in hematopoietic cells and is most homologous to the focal adhesion protein, paxillin. It may function in cell type-specific signaling by associating with PYK2, a member of focal adhesion kinase family. As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by tyrosine kinase activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LPXN