Leucyl-cystinyl Aminopeptidase/LNPEP Antibody (SP15)

Product Discontinued

Leucyl-cystinyl Aminopeptidase/LNPEP Antibody (SP15) Summary

• Immunogen: Recombinant protein encoding human placental alkaline phosphatase
• Localization: Cytoplasmic
• Specificity: This antibody reacts with a membrane-bound isoenzyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. This antibody is highly specific to PLAP and shows no cross-reaction with other isoenzymes of alkaline phosphatase. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA). This antibody cross-reacts with Human, Mouse, and Rat.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LNPEP
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin Neat
• Application Notes: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded and acetone fixed cryostat tissue sections. No further titration is required. "Intended Use: This product is intended for qualitative immunohistochemistry with normal and neoplastic formalin-fixed, paraffin-embedded tissue sections, to be viewed by light microscopy. Clinical interpretation of staining results should be accompanied by histological studies with proper controls. Patients? clinical histories and other relevant diagnostic tests should be utilized by a qualified person(s) when evaluating and interpreting results. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. Optimal incubation conditions should be determined by the user based upon the fixation conditions and staining system employed. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to immunostaining." This product has a prediluted size available. If a 6mL or 7mL pre-diluted size is purchased this product can be used Neat (without further dilution). Use recommended dilution for the concentrated vials only.

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Tissue culture supernatant
• Preservative: Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Leucyl-cystinyl Aminopeptidase/LNPEP Antibody (SP15)

• angiotensin IV receptor
• AT (4) receptor
• CAP
• Cystinyl aminopeptidase
• EC 3.4.11
• EC 3.4.11.3
• Insulin-regulated membrane aminopeptidase
• Insulin-responsive aminopeptidase
• IRAP
• leucyl/cystinyl aminopeptidase
• Leucylcystinyl Aminopeptidase
• Leucyl-cystinyl Aminopeptidase
• LNPEP
• Otase
• Oxytocinase
• Placental leucine aminopeptidase
• PLAP
• P-LAP
• P-LAPinsulin-regulated aminopeptidase
• Vasopressinase

Background

Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LNPEP