LEPRE1 Antibody - BSA Free

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Orthogonal Strategies: Western Blot: LEPRE1 Antibody [NBP2-30424] - Analysis in human cell lines U2OS and MCF-7 using anti-P3H1 antibody. Corresponding P3H1 RNA-seq data are presented for the same cell lines. ...read more
Immunohistochemistry-Paraffin: LEPRE1 Antibody [NBP2-30424] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: LEPRE1 Antibody [NBP2-30424] - Staining of human cervix shows weak to moderate cytoplasmic positivity in squamous epithelial cells.
LEPRE1 Antibody [NBP2-30424] - staining of human placenta shows strong cytoplasmic positivity in decidual cells.
LEPRE1 Antibody [NBP2-30424] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
LEPRE1 Antibody [NBP2-30424] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Validated by:
       

Orthogonal Strategies

 

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Catalog# & Formulation Size Price

LEPRE1 Antibody - BSA Free Summary

Description
Novus Biologicals Rabbit LEPRE1 Antibody - BSA Free (NBP2-30424) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: AVECAKTYLLFFPNDEVMNQNLAYYAAMLGEEHTRSIGPRESAKEYRQRSLLEKELLFFAYDVFGIPFVDPDSWTPEEVIPKRLQEKQKSERETAVRISQEIGNLMKEIETLVEEKTKESLDVSRLT
Predicted Species
Mouse (91%), Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
P3H1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
LEPRE1 Protein (NBP2-30424PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for LEPRE1 Antibody - BSA Free

  • EC 1.14.11.7
  • GROS1OI8
  • Growth suppressor 1
  • leprecan
  • Leprecan-1
  • Leucine- and proline-enriched proteoglycan 1
  • leucine proline-enriched proteoglycan (leprecan) 1
  • MGC117314
  • P3H1LEPRECAN
  • prolyl 3-hydroxylase 1

Background

LEPRE1 encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol P3H1
Uniprot