Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]

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Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse Legumain/Asparaginyl ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Legumain
Val18-Tyr435
Accession # O89017
Specificity
Detects mouse Legumain in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Legumain is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
LGMN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
Publications
Read Publications using
AF2058 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]

  • AEP
  • Asparaginyl Endopeptidase
  • cysteine protease 1
  • Legumain
  • LGMN
  • LGMN1
  • Protease, cysteine 1
  • protease, cysteine, 1 (legumain)
  • PRSC1EC 3.4.22.34

Background

Legumain is a lysosomal cysteine protease found in all mouse tissues examined, but was particularly abundant in kidney and placenta (1). Legumain plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases (2). Over-expression of Legumain in tumors is significant for invasion/metastasis (3). Also known as asparaginyl endopeptidase, it specifically cleaves peptide bonds with Asn at the P1 position. Nevertheless, it also cleaves peptide bonds with Asp at the P1 position. Auto-activation of pro Legumain involves both types of cleavage, which results in the removal of the pro peptides in both C- and N-termini (4). In addition, Legumain activates pro MMP-2 and processes bacterial antigens for MHC class II presentation and pro thymosin alpha to thymosin alpha 1 and thymosin alpha 11, two acidic peptides with immunoregulatory properties (5-7).

  1. Chen, J.M. et al. (1998) Biochem. J. 335:111.
  2. Shirahama-Noda, K. et al. (2003) J. Biol. Chem. 278:33194.
  3. Liu, C. et al. (2003) Cancer Res. 63: 2957.
  4. Li D.N. et al. (2003) J. Biol. Chem. 278:38980.
  5. Chen, J.M. et al. (2001) Biol. Chem. 382:777.
  6. Schwarz, G. et al. (2002) Biol. Chem. 383:1813.
  7. Sarndeses, C.S. et al. (2003) J. Biol. Chem. 278:13286.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Legumain/Asparaginyl Endopeptidase Antibody (AF2058)(4)

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Bioinformatics

Gene Symbol LGMN
Uniprot