Legumain/Asparaginyl Endopeptidase Antibody [Alexa Fluor™ Plus 488]

• Reactivity: Mu
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor Plus 488

Legumain/Asparaginyl Endopeptidase Antibody [Alexa Fluor™ Plus 488] Summary

• Specificity: Detects mouse Legumain in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Legumain/Asparaginyl Endopeptidase Antibody [Alexa Fluor™ Plus 488]

• AEP
• Asparaginyl Endopeptidase
• cysteine protease 1
• Legumain
• LGMN
• LGMN1
• Protease, cysteine 1
• protease, cysteine, 1 (legumain)
• PRSC1EC 3.4.22.34

Background

Legumain is a lysosomal cysteine protease found in all mouse tissues examined, but was particularly abundant in kidney and placenta (1). Legumain plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases (2). Over-expression of Legumain in tumors is significant for invasion/metastasis (3). Also known as asparaginyl endopeptidase, it specifically cleaves peptide bonds with Asn at the P1 position. Nevertheless, it also cleaves peptide bonds with Asp at the P1 position. Auto-activation of pro Legumain involves both types of cleavage, which results in the removal of the pro peptides in both C- and N-termini (4). In addition, Legumain activates pro MMP-2 and processes bacterial antigens for MHC class II presentation and pro thymosin alpha to thymosin alpha ₁ and thymosin alpha ₁₁, two acidic peptides with immunoregulatory properties (5-7).

1. Chen, J.M. et al. (1998) Biochem. J. 335:111.
2. Shirahama-Noda, K. et al. (2003) J. Biol. Chem. 278:33194.
3. Liu, C. et al. (2003) Cancer Res. 63: 2957.
4. Li D.N. et al. (2003) J. Biol. Chem. 278:38980.
5. Chen, J.M. et al. (2001) Biol. Chem. 382:777.
6. Schwarz, G. et al. (2002) Biol. Chem. 383:1813.
7. Sarndeses, C.S. et al. (2003) J. Biol. Chem. 278:13286.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.