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HAF109). Specific bands were detected for Pro-Legumain/ Asparaginyl Endopeptidase at approximately 56 kDa and mature Legumain/Asparaginyl Endopeptidase 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
2199-CY) was serially diluted and captured by Mouse Anti-Human Legumain/Asparaginyl Endopeptidase Monoclonal Antibody (Catalog # MAB21992) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Legumain/Asparaginyl Endopeptidase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2199) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
HAF005). A specific band was detected for Legumain/Asparaginyl Endopeptidase at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
Legumain encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq]
![Western Blot Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Legumain_AF2199_Western_Blot_11805.jpg)
![Western Blot Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]](http://images.novusbio.com/fullsize/antibody/Legumain_AF2058_Western_Blot_17886.jpg)
![Western Blot Legumain/Asparaginyl Endopeptidase Antibody (301417) [Unconjugated]](http://images.novusbio.com/images/antibody/Legumain_MAB2058_Western_Blot_17942.jpg)
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![Western Blot Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2199_human-legumain-affinity-purified-polyclonal-ab-41202412403525.jpg)
![Immunocytochemistry/ Immunofluorescence Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2199_human-legumain-affinity-purified-polyclonal-ab-41202412401277.jpg)
![Immunohistochemistry Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2199_human-legumain-affinity-purified-polyclonal-ab-41202412394999.jpg)
![Western Blot Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2199_human-legumain-affinity-purified-polyclonal-ab-4120241240126.jpg)
![Immunocytochemistry/ Immunofluorescence Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2199_human-legumain-affinity-purified-polyclonal-ab-41202412401240.jpg)
![Immunocytochemistry/ Immunofluorescence Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2199_human-legumain-affinity-purified-polyclonal-ab-41202412403597.jpg)
![Western Blot Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2199_human-legumain-affinity-purified-polyclonal-ab-41202412394927.jpg)
![Western Blot Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2199_human-legumain-affinity-purified-polyclonal-ab-4120241240241.jpg)
![Immunohistochemistry Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2199_human-legumain-affinity-purified-polyclonal-ab-22820241723645.jpg)
![ELISA Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]](http://images.novusbio.com/images/af2199_human-legumain-affinity-purified-polyclonal-ab-elisa-639201854768224563.jpg)
![Western Blot Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2058_mouse-legumain-affinity-purified-polyclonal-ab-western-blot-121220258463413.jpg)
