Legumain/Asparaginyl Endopeptidase Antibody (S06-7G1)

Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (S06-7G1) [NBP3-19561] - Western blot detection of Jurkat, Hela cell lysates using NBP3-19561. Predicted band size: 49kDa. Observed band size: 56, 46, 37kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S06-7G1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Legumain/Asparaginyl Endopeptidase Antibody (S06-7G1) Summary

• Description: Novus Biologicals Rabbit Legumain/Asparaginyl Endopeptidase Antibody (S06-7G1) (NBP3-19561) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human Legumain/Asparaginyl Endopeptidase (Uniprot # Q99538)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LGMN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4), 150mM sodium chloride, 0.05% BSA, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Legumain/Asparaginyl Endopeptidase Antibody (S06-7G1)

• AEP
• Asparaginyl Endopeptidase
• cysteine protease 1
• Legumain
• LGMN
• LGMN1
• Protease, cysteine 1
• protease, cysteine, 1 (legumain)
• PRSC1EC 3.4.22.34

Background

Legumain encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LGMN