Legumain/Asparaginyl Endopeptidase Antibody (312109)

• Reactivity: Hu
• Applications: WB, ELISA(Cap)
• Clone: 312109
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Legumain/Asparaginyl Endopeptidase Antibody (312109) Summary

• Immunogen: NS0-derived recombinant human Legumain
  Val18-Tyr433
  Accession # Q99538
• Specificity: Detects both the pro and mature forms of human Legumain/Asparaginyl Endopeptidase in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LGMN
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human Legumain/Asparaginyl Endopeptidase Biotinylated Antibody (Catalog number BAF2199)
  Standard: Recombinant Human Legumain/Asparaginyl Endopeptidase (Catalog number 2199-CY)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Legumain/Asparaginyl Endopeptidase Antibody (312109)

• AEP
• Asparaginyl Endopeptidase
• cysteine protease 1
• Legumain
• LGMN
• LGMN1
• Protease, cysteine 1
• protease, cysteine, 1 (legumain)
• PRSC1EC 3.4.22.34

Background

Legumain is a lysosomal cysteine protease whose activity is found in several tissues tested (1, 2). Legumain plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases (3). Over-expression of Legumain in tumors is significant for invasion/metastasis (4). Also known as Asparaginyl Endopeptidase, it specifically cleaves peptide bonds with Asn at the P1 position. Nevertheless, it also cleaves peptide bonds with Asp at the P1 position. Auto-activation of pro Legumain involves both types of the cleavage, which result in the removal of the pro peptides in both C- and N-termini (5). In addition, Legumain activates pro MMP-2 and processes bacterial antigens for MHC class II presentation and pro thymosin alpha to thymosin alpha ₁ and thymosin alpha ₁₁, two acidic peptides with immunoregulatory properties (6‑8). Human Legumain is synthesized as a 433 amino acid precursor with a signal peptide (residues 1‑17). The pro enzyme (residues 18‑433) was expressed with an N-terminal His tag. This activity of Legumain can be inhibited by rhCystatins C and E/M and recombinant mouse Cystatin C (R&D Systems, Catalog # 1196-PI, 1286-PI and 1238-PI, respectively).

1. Chen, J.-M. et al. (1997) J. Biol. Chem. 272:8090.
2. Tanaka, T. et al. (1996) Cytogenet. Cell Genet. 74:120.
3. Shirahama-Noda, K. et al. (2003) J. Biol. Chem. 278:33194.
4. Liu, C. et al. (2003) Cancer Res. 63: 2957.
5. Li D.N. et al. (2003) J. Biol. Chem. 278:38980.
6. Chen, J.M. et al. (2001) Biol. Chem. 382:777.
7. Schwarz, G. et al. (2002) Biol. Chem. 383:1813.
8. Sarndeses, C.S. et al. (2003) J. Biol. Chem. 278:13286.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Legumain/Asparaginyl Endopeptidase Antibody (MAB21992)(1)

Publication using MAB21992

• Smith R, Solberg R, Jacobsen L, Voreland A, Rustan A, Thoresen G, Johansen H Simvastatin inhibits glucose metabolism and legumain activity in human myotubes. PLoS ONE, 2014-01-08;9(1):e85721. 2014-01-08 [PMID: 24416446] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: LGMN
• Uniprot:
  • Q99538()