LEF1 Antibody (REMB1)

Product Discontinued

LEF1 Antibody (REMB1) Summary

• Immunogen: Recombinant full length LEF1 (Human).
• Localization: Nuclear
• Specificity: Recognizes the beta-Catenin Binding Domain of human LEF1 using immunoblotting (55-57 kDa). The does not cross-react with TCF1 or TCF4.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LEF1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: IF: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 5 ug/ml, this is recommended using whole extracts of human Jurkat cells or transiently expressed human LEF1 in whole cell lysates from Cos-1 cells. Predicted MW 55-57 kDa.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.5)
• Preservative: 0.08% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for LEF1 Antibody (REMB1)

• DKFZp586H0919
• FLJ46390
• LEF1
• LEF-1
• lymphoid enhancer-binding factor 1
• T cell-specific transcription factor 1-alpha
• TCF10
• TCF1ALPHA
• TCF1-alpha
• TCF7L3

Background

Lymphoid enhancer factor, LEF1, is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF1, TCF1, TCF3 and TCF4). These factors play crucial roles in WNT/Wingless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. LEF1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF1 (for T cell Factor 1) for correct development of T lymphocytes in the thymus. LEF1 is a 399 amino acid protein. Alternative splice forms of LEF1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on immunoblots as small immunoreactive forms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LEF1
• Entrez:
  • 51176(Human)