LEF1 Antibody (1C3)

Western Blot: LEF1 Antibody (1C3) [NBP2-66625] - Analysis using LEF-1 (alternate exon) antibody (Cat. No.NBP2-66625) on Cos cells transfected with the indicated proteins.

Product Discontinued

LEF1 Antibody (1C3) Summary

• Immunogen: Recombinant full-length LEF1 protein
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LEF1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Detect LEF-1 protein containing the alternately spliced exon between amino acids 212-241 by Western blot at 1-5 ug/ml. Optimal concentration should be evaluated by serial dilutions..

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.08% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for LEF1 Antibody (1C3)

• DKFZp586H0919
• FLJ46390
• LEF1
• LEF-1
• lymphoid enhancer-binding factor 1
• T cell-specific transcription factor 1-alpha
• TCF10
• TCF1ALPHA
• TCF1-alpha
• TCF7L3

Background

Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody is specific for an epitope between a.a. 227-231. This sequence lies within the context dependent activation domain of LEF-1 and appears to be an alternate splice variant exon of the LEF-1 gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LEF1
• Uniprot:
  • Q9UJU2(Human)