Lck [p Ser192] Antibody

Product Discontinued

Lck [p Ser192] Antibody Summary

• Immunogen: Synthetic peptide (Human) derived from the region of human Lck that contains tyrosine 192 (based on Swiss Protein database, accession number P06239). The sequence is conserved in human and mouse.
• Modification: p Ser192
• Localization: Cytoplasmic
• Specificity: Lck (79% identity), Hck (79% identity) and Lyn (93% identity) have not been tested.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LCK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1-1.0 ug/ml
• Application Notes: Western Blot Predicted molecular weight 60 kDa.

Reactivity Notes

Cross-reacts with Human. Expected to cross-react with Chicken (79% identity with immunogen), Mouse (100% identity with immunogen) due to sequence homology.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Dulbecco's PBS (pH 7.3), 1.0 mg/ml and 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Lck [p Ser192] Antibody

• EC 2.7.10
• EC 2.7.10.2
• Hck-3
• Lck
• Lcktkr
• Leukocyte C-terminal Src kinase
• LSK
• LSTRA
• Lymphocyte cell-specific protein-tyrosine kinase
• lymphocyte-specific protein tyrosine kinase
• p56(LSTRA) protein-tyrosine kinase
• p56lck
• p56-LCK
• pp58lck
• Protein YT16
• Proto-oncogene Lck
• proto-oncogene tyrosine-protein kinase LCK
• T cell-specific protein-tyrosine kinase
• T-lymphocyte specific protein tyrosine kinase p56lck
• tyrosine-protein kinase Lck
• YT16

Background

Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is regulated by phosphorylation of multiple sites, including the two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src). Phosphorylation of Lck Tyr-192 blocks high affinity binding of other proteins to the SH2 domain of Lck, thereby negatively regulating its participation in T cell antigen receptor signalling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LCK
• Entrez:
  • 3932(Human)