LC3A Antibody (2312D) [Biotin]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
2312D
Clonality
Monoclonal
Host
Rabbit
Conjugate
Biotin

LC3A Antibody (2312D) [Biotin] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Partial synthetic peptide from the C-terminal of human LC3A [UniProt Q9H492]
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MAP1LC3A
Purity
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from cell culture supernatant

Alternate Names for LC3A Antibody (2312D) [Biotin]

  • Apg8
  • APG8a
  • Apg8p3
  • ATG8E
  • Autophagy-related protein LC3 A
  • Autophagy-related ubiquitin-like modifier LC3 A
  • LC3
  • LC3A
  • lc3-i, ii
  • MAP1A/1B light chain 3 A
  • MAP1ALC3
  • MAP1ALC3MAP1A/MAP1B LC3 A
  • MAP1BLC3MAP1A/MAP1B light chain 3 A
  • MAP1LC3A
  • microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1
  • microtubule-associated proteins 1A/1B light chain 3
  • microtubule-associated proteins 1A/1B light chain 3A
  • MLP3A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LC3A Antibody (NBP2-75924B). (Showing 1 - 1 of 1 FAQ).

  1. May we ask if it is possible to perform IF to stain LC3-I and LC3-II separately with two different fluorescent colors?
    • Yes, it is possible to perform IF stain for LC3-I and LC3-II separately with two different fluorescent colors! You will have to use two different primary antibodies and in order to avoid any potential background/cross reactivity issues, I would suggest that you employ conjugated primary antibodies for the testing. 1. Our LC3I antibody (NBP1-78964) has been designed to specifically detect the cytosolic form of the LC3 protein which is actually LC3 I (Note: LC3-II binds to the autophagic membranes). 2. There is not even a single antibody to our knowledge that would exclusively detect the LC3 II form, and you would have to detect LC3II/ autophagic membranes form with an antibody which detects LC3 I/LC3II together. Therefore you may opt second antibody from one of the followings: LC3 Antibody (NB100-2220), LC3 Antibody (NB100-2331), LC3 Antibody (NBP1-19167). All of these mentioned catalog #s come with different options for their conjugated forms and you may select appropriate conjugated forms for performing the IF staining using our explained criteria.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-75924AF350
Alexa Fluor 405 NBP2-75924AF405
Alexa Fluor 488 NBP2-75924AF488
Alexa Fluor 532 NBP2-75924AF532
Alexa Fluor 594 NBP2-75924AF594
Alexa Fluor 647 NBP2-75924AF647
Alexa Fluor 700 NBP2-75924AF700
Alexa Fluor 750 NBP2-75924AF750
Biotin NBP2-75924B
DyLight 350 NBP2-75924UV
DyLight 405 NBP2-75924V
DyLight 488 NBP2-75924G
DyLight 550 NBP2-75924R
DyLight 594 NBP2-75924DL594
DyLight 650 NBP2-75924C
DyLight 680 NBP2-75924FR
DyLight 755 NBP2-75924IR
FITC NBP2-75924F
HRP NBP2-75924H
Janelia Fluor 549 NBP2-75924JF549
Janelia Fluor 646 NBP2-75924JF646

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Bioinformatics Tool for LC3A Antibody (NBP2-75924B)

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Blogs on LC3A.


  Read full blog post.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
By Jamshed Arslan Pharm.D. Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amyloid beta (a polypepti...  Read full blog post.

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Bioinformatics

Gene Symbol MAP1LC3A