Human LBP ELISA Kit (Colorimetric)

Product Discontinued

Human LBP ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 1.5 to 50 ng/mL
• Assay Type: Sandwich ELISA
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: LBP

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: LBP (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human LBP.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human LBP ELISA Kit (Colorimetric)

• LBP
• lipopolysaccharide binding protein
• lipopolysaccharide-binding protein
• LPS-binding protein
• MGC22233

Background

The protein encoded by the LBP gene is involved in the acute-phase immunologic response to gram-negative bacterialinfections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Togetherwith bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that theencoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS fromcirculation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasmacholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). Finally, this gene is found onchromosome 20, immediately downstream of the BPI gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for LBP ELISA Kit (KA0448)(17)

Publications using KA0448

• Guillen Y, Noguera-Julian M, Rivera J et al. Low nadir CD4+ T-cell counts predict gut dysbiosis in HIV-1 infection. Mucosal Immunol 2018-08-31 [PMID: 30171206]
• gler H, Elsenbruch S, Rebernik L et al. Stress burden and neuroendocrine regulation of cytokine production in patients with ulcerative colitis in remission. Psychoneuroendocrinology 2018-08-07 [PMID: 30125791]
• Kim KE, Heo JS, Han S et al. Blood concentrations of lipopolysaccharide-binding protein, high-sensitivity C-reactive protein, tumor necrosis factor-?, and Interleukin-6 in relation to insulin resistance in young adolescents. Clin Chim Acta 2018-07-29 [PMID: 30059659]
• Yang Y, Qu C, Liang S et al. Estrogen inhibits the overgrowth of Escherichia coli in the rat intestine under simulated microgravity. Mol Med Rep 2017-11-20 [PMID: 29207065]
• Trojova I, Kozarova M, Petrasova D et al. Circulating lipopolysaccharide-binding protein and carotid intima-media thickness in obstructive sleep apnea. Physiol Res 2017-11-10 [PMID: 29137477]
• Nier A, Engstler AJ, Maier IB et al. Markers of intestinal permeability are already altered in early stages of non-alcoholic fatty liver disease: Studies in children. PLoS One 2017-09-07 [PMID: 28880885]
• Milan AM, Pundir S, Pileggi CA et al. Comparisons of the Postprandial Inflammatory and Endotoxaemic Responses to Mixed Meals in Young and Older Individuals: A Randomised Trial. Nutrients 2017-04-02 [PMID: 28368340]
• Loganes C, Pin A, Naviglio S et al. Altered pattern of tumor necrosis factor-alpha production in peripheral blood monocytes from Crohn's disease. World J Gastroenterol 2016-11-07 [PMID: 27895399]
• Brinkmann C, Schulte-Korne B, Grau M et al. Effects of Endurance Training on the Skeletal Muscle Nitric Oxide Metabolism in Insulin-Independent Type 2 Diabetic Men-A Pilot Study. Metab Syndr Relat Disord 2016-10-26 [PMID: 27782779]
• Crapser J, Ritzel R, Verma R et al. Ischemic stroke induces gut permeability and enhances bacterial translocation leading to sepsis in aged mice. Aging (Albany NY) 2016-04-25 [PMID: 27115295]
• Liu M, Xu Y, Han X et al. Dioscin alleviates alcoholic liver fibrosis by attenuating hepatic stellate cell activation via the TLR4/MyD88/NF-kB signaling pathway. Sci Rep 2015-12-10 [PMID: 26655640]
• Engler H, Benson S, Wegner A et al. Men and women differ in inflammatory and neuroendocrine responses to endotoxin but not in the severity of sickness symptoms. Brain Behav Immun. 2015-08-17 [PMID: 26291403]
• Kheirandish-Gozal L, Peris E, Wang Y et al. Lipopolysaccharide-binding protein plasma levels in children: effects of obstructive sleep apnea and obesity. J Clin Endocrinol Metab 2014-02-01 [PMID: 24276451]
• Plessis JD, Vanheel H, Janssen CE et al. Activated intestinal macrophages in patients with cirrhosis release NO and IL-6 that may disrupt intestinal barrier function. J Hepatol. 2013-01-25 [PMID: 23402745]
• Reiberger T, Ferlitsch A, Payer BA et al. Nonselective betablocker therapy decreases intestinal permeability and serum levels of LBP and IL-6 in patients with cirrhosis. J Hepatol. 2012-12-19 [PMID: 23262249]
• Berner R, Furll B, Stelter F et al. Elevated levels of lipopolysaccharide-binding protein and soluble CD14 in plasma in neonatal early-onset sepsis. Clin Diagn Lab Immunol. 2002-03-01 [PMID: 11874891]
• Schroedl W, Fuerll B, Reinhold P et al. A novel acute phase marker in cattle: lipopolysaccharide binding protein (LBP). J Endotoxin Res. 2001-01-01 [PMID: 11521082]

FAQs for LBP ELISA Kit (KA0448). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: LBP