Lass5 Antibody - BSA Free

Western Blot: Lass5 Antibody [NBP1-77329] - Rat brain tissue lysate with LASS5 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry: Lass5 Antibody - BSA Free [NBP1-77329] - Immunohistochemistry of Lass5 in human brain tissue with Lass5 antibody at 2.5 u/mL.

Immunocytochemistry/ Immunofluorescence: Lass5 Antibody - BSA Free [NBP1-77329] - Immunofluorescence of Lass5 in Human Brain cells with Lass5 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Lass5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Lass5 Antibody - BSA Free (NBP1-77329) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of the human LASS5. The immunogen is located within amino acids 90 - 140 of LASS5. Amino Acid Squence: KYPDKKRLEGLSKQLD
• Specificity: Multiple isoforms of LASS5 are known to exist. This antibody may cross-react with the highly homologous LASS6.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CERS5
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  Lass5 Peptide (NBP1-77329PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Lass5 Antibody - BSA Free

• CerS5
• FLJ25304
• LAG1 homolog, ceramide synthase 5
• LAG1 longevity assurance homolog 5 (S. cerevisiae)
• LAG1 longevity assurance homolog 5
• MGC45411
• Trh4

Background

The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. LASS5, also called Trh4, is a 392 amino acid endoplasmic reticulum, multi-pass membrane protein. Functioning as a dihydro-ceramide synthase, LASS5 is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked palmitoyl-ceramide) in a fumonisin B1-independent manner. It uses palmitoyl-CoA as an acyl donor and is involved in the synthesis of C14, C16 and C18-ceramide. LASS5 is the most abundantly expressed and predominant ceramide synthase isoform in lung epithelia. Recent studies show that LASS5 partially correct growth and apoptosis. Multiple isoforms of LASS5 are known to exist. This antibody may cross-react with the highly homologous LASS6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CERS5
• Uniprot:
  • NP_671723(Human)