LASS2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: LASS2 Antibody [NBP1-84537] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane & endoplasmic reticulum. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: LASS2 Antibody [NBP1-84537] - Staining of human skeletal muscle shows only weak cytoplasmic positivity in striated muscle fibers as expected.

Immunohistochemistry-Paraffin: LASS2 Antibody [NBP1-84537] - Staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: LASS2 Antibody [NBP1-84537] - Staining of human liver shows moderate to strong granular cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: LASS2 Antibody [NBP1-84537] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Analysis in human cell line PC-3.

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LASS2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LASS2 Antibody - BSA Free (NBP1-84537) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-LASS2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TPLAALLNIKEKTRLRAPPNATLEHFYLTSGKQPKQVEVELLSRQSGLSGRQVARWFRRRRNQDRPSLLKKFREA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CERS2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  LASS2 Protein (NBP1-84537PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for LASS2 Antibody - BSA Free

• ceramide synthase 2
• CerS2
• LAG1 homolog, ceramide synthase 2
• LAG1 longevity assurance 2
• LAG1 longevity assurance homolog 2 (S. cerevisiae)
• LAG1 longevity assurance homolog 2
• S. cerevisiae) homolog 2
• SP260MGC987
• TMSG1
• Tumor metastasis-suppressor gene 1 protein

Background

LASS2 encodes a protein that has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein may play a role in the regulation of cell growth. Alternatively spliced transcript variants encoding the same protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LASS2 Antibody (NBP1-84537)(1)

Publication using NBP1-84537

• Torrino S, Tiroille V, Dolfi B Et al. UBTD1 regulates ceramide balance and endolysosomal positioning to coordinate EGFR signaling eLife 2021-04-22 [PMID: 33884955] (WB)

Bioinformatics

• Gene Symbol: CERS2