LASS1 Antibody

Western Blot: LASS1 Antibody [NBP1-28480] - Staining of Frontal Cortex lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Po, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LASS1 Antibody Summary

• Immunogen: Peptide with sequence C-KDLREYDTAEAQ corresponding to internal region according to NP_937850.1.
• Epitope: C terminus
• Specificity: This antibody is expected to recognize both reported isoforms (NP_067090.1and NP_937850.1).
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CERS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx. 37 kDa band observed in human brain (cerebellum, cerebral cortex and frontal cortex) lysates (calculated MW of 38.0 kDa band according to NP_937850.1). An additional band of unknown identity was also consistently observed at 85 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LASS1 Antibody

• CerS1
• LAG1 homolog, ceramide synthase 1
• LAG1 longevity assurance homolog 1
• LAG1MGC90349
• longevity assurance (LAG1, S. cerevisiae) homolog 1
• Longevity assurance gene 1 protein homolog 1
• Protein UOG-1
• UOG1LAG1 longevity assurance homolog 1 (S. cerevisiae)
• upstream of GDF1

Background

This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site that is cleaved to produce a mature protein containing seven conserved cysteine residues. Members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in yeast suggest that the encoded protein is involved in aging. This protein is transcribed from a monocistronic mRNA as well as a bicistronic mRNA, which also encodes growth differentiation factor 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LASS1 Antibody (NBP1-28480)(2)

Publications using NBP1-28480

• Kim JE, Kwon EY, Han Y Allulose Attenuated Age-Associated Sarcopenia via Regulating IGF-1 and Myostatin in Aged Mice Molecular nutrition & food research 2021-10-28 [PMID: 34710274] (WB, Mouse)
• Venkataraman K, Riebeling C, Bodennec J et al. Upstream of growth and differentiation factor 1 (uog1), a mammalian homolog of the yeast longevity assurance gene 1 (LAG1), regulates N-stearoyl-sphinganine (C18-(dihydro)ceramide) synthesis in a fumonisin B1-independent manner in mammalian cells. J Biol Chem 2002-09-20 [PMID: 12105227]

Bioinformatics

• Gene Symbol: CERS1
• Entrez:
  • 10715(Human)
  • 93898(Mouse)
  • 306351(Rat)
• Uniprot:
  • P27544(Human)