Lano Antibody - BSA Free

Western Blot: Lano Antibody [NBP1-76678] - Analysis of Lano expression in A431 cell lysate with Lano antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry: Lano Antibody - BSA Free [NBP1-76678] - Immunohistochemistry of Lano in human colon tissue with Lano antibody at 10 ug/mL.

Immunocytochemistry/ Immunofluorescence: Lano Antibody - BSA Free [NBP1-76678] - Immunofluorescence of Lano in human colon tissue with Lano antibody at 20 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Lano Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Lano Antibody - BSA Free (NBP1-76678) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human Lano. The immunogen is located within the last 50 amino acids of Lano. Amino Acid Squence: KNEVNHAIDRVTTSV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LRRC1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Lano Peptide (NBP1-76678PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Lano Antibody - BSA Free

• dJ523E19.1
• FLJ10775
• FLJ11834
• LANO
• LANOLANO adapter protein
• LAP (leucine-rich repeats and PDZ) and no PDZ protein
• LAP and no PDZ protein
• leucine rich repeat containing 1
• leucine-rich repeat-containing 1
• leucine-rich repeat-containing protein 1
• LRRC1

Background

Lano is a member of the LAP (leucine-rich repeats and PDZ) family of proteins that also includes Densin-180, Erbin, and hScribble. The LAP proteins generally contain multiple leucine-rich repeat (LRR) domains which serve to target them to the basolateral membrane of epithelial cells. Lano is unique in that it alone does not possess one or more PDZ (PSD95/DLG/ZO-1) domains as do the other members of the LAP family. However, it can bind to the PDZ domain of Erbin in addition to those of membrane-associated and guanylate kinase (MAGUK) proteins which regulate adhesion and plasticity at cell junctions. It has been suggested that it is through these interaction that these LAP proteins participate in the maintenance of proper embryonic development and integrity of epithelial tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRRC1
• Uniprot:
  • AAK72246(Human)