Rat Laminin S/Laminin beta 2 ELISA Kit

Product Discontinued

Rat Laminin S/Laminin beta 2 ELISA Kit Summary

• Immunogen: Laminin is a large (900 kDa) complex extracellular glycoprotein and a major component of the basement membrane. Laminin is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin is composed of three different polypeptide chains; alpha, beta and gamma which are bound to each other by disulphide bonds into a cross-shaped molecule. Isoforms have been detected for all three chains. The different combinations of the alpha, beta and gamma chains give rise to at least 15 laminin heterotrimers.
• Marker: Schwann Cell Marker
• Specificity: Rat Laminin
• Kit Type: ELISA Kit
• Gene: LAMB2

Applications/Dilutions

• Dilutions:
  • ELISA 156pg/ml-10000pg/ml
• Application Notes: This product is useful for Sandwich ELISA detection. Strip format Biosensis ELISA kits offer the highest level of sensitivity available Strip 96-well format, use only what you need: High sensitivity and low blank Suitability for serum & tissues Reproducible results This kit contains: Pre-coated plates, Prepared standards Buffers, Detection reagents, Substrate Precise methods/instructions.

Reactivity Notes

Rat

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

This kit contains 2.0 x 96.0 Well Strip.

Alternate Names for Rat Laminin S/Laminin beta 2 ELISA Kit

• LAMB2
• Laminin B1s chain
• Laminin beta 2
• Laminin S
• laminin subunit beta-2
• laminin, beta 2 (laminin S)
• Laminin-11 subunit beta
• Laminin-14 subunit beta
• Laminin-15 subunit beta
• Laminin-3 subunit beta
• Laminin-4 subunit beta
• Laminin-7 subunit beta
• LAMSLaminin-9 subunit beta
• S-LAM beta
• S-laminin subunit beta

Background

The rat Laminin Kit is a sandwich ELISA. The capture antibody is a polyclonal rat Laminin antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known Laminin concentration are added to these wells and allowed to complex with the bound Laminin antibody. A biotinylated rat Laminin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Laminin present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat Laminin in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Laminin S/Laminin beta 2 ELISA Kit (BEK-2055-2P). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: LAMB2