Laminin beta 1 Antibody

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Immunohistochemistry: Laminin beta 1 Antibody [NB120-6571] - Tissue: skin. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-Laminin at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit ...read more
Immunohistochemistry: Laminin beta 1 Antibody [NB120-6571] - Tissue: Placenta. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Incubated at 5 ug/mL for 1 h at RT.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Laminin beta 1 Antibody Summary

Immunogen
Produced against a mixture of synthetic peptides corresponding to regions of adherence on laminin.
Localization
Extracellular. Found in the basement membranes.
Marker
Schwann Cell Marker
Specificity
Negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LAMB1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:4000-1:8000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
Application Notes
This product was assayed by immunoblot and found to be reactive against Laminin at a dilution of 1:5,000 - 1:10,000. This product was also assayed against 1.0 ug of Laminin in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:4,000 - 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 - 1:200.

Reactivity Notes

Cross-reacts with most Mammals.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.125M Sodium Borate, 75mM NaCl and 5mM EDTA, pH 8.0
Preservative
0.01% Sodium Azide
Purity
IgG purified

Alternate Names for Laminin beta 1 Antibody

  • CLM
  • cutis laxa with marfanoid phenotype
  • Laminin B1 chain
  • laminin subunit beta-1
  • laminin, beta 1
  • Laminin-1 subunit beta
  • Laminin-10 subunit beta
  • Laminin-12 subunit beta
  • Laminin-2 subunit beta
  • Laminin-6 subunit beta
  • Laminin-8 subunit beta
  • MGC142015

Background

Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with an approximate molecular weight of 900 kDa. It plays an important role in many aspects of the cell biology. Laminin is composed of one A chain (400 kDa) one B1 chain (215 kDa) and one B2 chain (205 kDa) all held together by disulfide bonds. The molecule has a cross like form with globular units near the ends of each chain, the sites where it is bound to Collagen IV, heparan sulfate, proteoglycan as well as to the surface of epithelial cells. Laminins from various species have common antigenic determinants. Laminin is only found in significant quantities in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. It has been found to modulate cell differentiation, cell shaping and also cell movement because it appears to be an important cell substrate adhesion protein. Variations in the expression of this protein have been observed in embryogenesis, organogenesis, post traumatic healing and cancer. The greatest interest in laminin has been provoked by the discovery of its ability to promote neurite regeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol LAMB1