Lamin B Receptor Overexpression Lysate (Native)

Images

 
Western Blot: Lamin B Receptor Overexpression Lysate (Adult Normal) [NBL1-12453] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lamin B Receptor.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Lamin B Receptor Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201990 and based on accession number NM_002296. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens lamin B receptor (LBR), transcript variant 1, mRNA.
Gene
LBR

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
70.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Lamin B Receptor

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Lamin B Receptor Overexpression Lysate (Native)

  • DHCR14B
  • FLJ43126
  • Integral nuclear envelope inner membrane protein
  • lamin B receptor
  • lamin-B receptor
  • LMN2R
  • MGC9041
  • PHA

Background

The protein encoded by this gene belongs to the ERG4/ERG24 family. It localized in the nuclear envelope inner membrane and anchors the lamina and the heterochromatin to the membrane. It may mediate interaction between chromatin and lamin B. Mutations of this gene has been associated with autosomal recessive HEM/Greenberg skeletal dysplasia. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-24867
Species: Hu, Mu
Applications: Flow, Func, In vitro, In vivo, CyTOF-ready, Flow-CS
NBP1-89366
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
H00027161-M01
Species: Hu, Mu, Rt
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP, RNAi
NBP2-56060
Species: Hu
Applications: ICC/IF
H00030813-R02
Species: Hu
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP1-26666
Species: Mu
Applications: B/N, Flow, IP, In vitro

Publications for Lamin B Receptor Lysate (NBL1-12453) (0)

There are no publications for Lamin B Receptor Lysate (NBL1-12453).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Lamin B Receptor Lysate (NBL1-12453) (0)

There are no reviews for Lamin B Receptor Lysate (NBL1-12453). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Lamin B Receptor Lysate (NBL1-12453) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Lamin B Receptor Products

Bioinformatics Tool for Lamin B Receptor Lysate (NBL1-12453)

Discover related pathways, diseases and genes to Lamin B Receptor Lysate (NBL1-12453). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Lamin B Receptor Lysate (NBL1-12453)

Discover more about diseases related to Lamin B Receptor Lysate (NBL1-12453).
 

Pathways for Lamin B Receptor Lysate (NBL1-12453)

View related products by pathway.

PTMs for Lamin B Receptor Lysate (NBL1-12453)

Learn more about PTMs related to Lamin B Receptor Lysate (NBL1-12453).

Blogs on Lamin B Receptor

There are no specific blogs for Lamin B Receptor, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Lamin B Receptor Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol LBR