LAIR1 Antibody (113) - BSA Free

Flow Cytometry: LAIR1 Antibody (113) [NBP1-43324] - Staining of C57BL/6 splenocytes with 0.125 ug of Armenian Hamster IgG Isotype Control Purified (purple line) or 0.125 ug of Anti-Mouse CD305 (LAIR-1) Purified (blue line) followed by Anti-Armenian Hamster IgG PE. Total viable F4/80+ cells were used for analysis.

• Reactivity: Mu
• Applications: WB, Flow, IP
• Clone: 113
• Clonality: Monoclonal
• Host: Armenian Hamster
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 5 mg/ml

LAIR1 Antibody (113) - BSA Free Summary

• Description: Novus Biologicals Armenian Hamster LAIR1 Antibody (113) - BSA Free (NBP1-43324) is a monoclonal antibody validated for use in WB, Flow and IP. Anti-LAIR1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody was CD305.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: LAIR1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blot
• Application Notes: This 113 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 5 mg/ml
• Purity: Protein A or G purified

Alternate Names for LAIR1 Antibody (113) - BSA Free

• CD305 antigen
• CD305
• CD305leukocyte-associated Ig-like receptor 1
• HLAIR1
• LAIR1
• LAIR-1
• leukocyte-associated immunoglobulin-like receptor 1

Background

CD305 or Leukocyte-associated Ig-like receptor-1 (LAIR-1) is a 46 kD cell surface molecule that functions as an inhibitory collagen-receptor on the majority of cells of the immune system. The mouse LAIR-1 molecule is expressed primarily on monocytes/macrophages and also on T cells, NK cells and dendritic cells. Unlike human B cells , mouse B cells do not express LAIR-1. The cytoplasmic tail of mouse LAIR-1 can be phosphorylated leading to the recruitment of SH2-containing tyrosine phosphatase(SHP)-2 and C-terminal Src kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LAIR1 Antibody (NBP1-43324)(1)

Publication using NBP1-43324

• Myers LK, Ouyang YX, Patel JR et al. Role of Citrullinated Collagen in Autoimmune Arthritis International journal of molecular sciences 2022-08-30 [PMID: 36077232] (WB, Mouse)

Bioinformatics

• Gene Symbol: LAIR1