AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " alt="PBMC lymphocytes were stained with Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Goat Anti-Human LAIR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # af2664) or (B) isotype control antibody (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2664-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84590-25ul | |||
HAF008). Specific bands were detected for LAIR1 at approximately 36-14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and mouse thymus tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Mouse LAIR1 Monoclonal Antibody (Catalog # MAB10092) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for LAIR1 at approximately 36-14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10092-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB100921-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43324-0.05mg | |||
| BSA Free | NBP1-43324-0.025mg | |||
MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="Human lymphocytes were stained with Mouse Anti-Human LAIR1 Monoclonal Antibody (Catalog # MAB2664, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
![Flow (Cell Surface): LAIR1 Antibody (NKTA255) [NBP2-62214] - Surface staining of CD305 / LAIR-1 in human peripheral blood with anti-CD305 (NKTA255) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/LAIR1-Antibody-NKTA255-Flow-Cell-Surface-NBP2-62214-img0002.jpg "Flow (Cell Surface): LAIR1 Antibody (NKTA255) [NBP2-62214] - Surface staining of CD305 / LAIR-1 in human peripheral blood with anti-CD305 (NKTA255) PerCP-CyTM5.5.")
![Flow (Cell Surface): LAIR1 Antibody (NKTA255) [NBP2-62214] - Surface staining of CD305 / LAIR-1 in human peripheral blood (lymphocyte gate) with anti-CD305 (NKTA255) PE.](http://images.novusbio.com/fullsize/LAIR1-Antibody-NKTA255-Flow-Cell-Surface-NBP2-62214-img0001.jpg)
![Immunohistochemistry-Paraffin: LAIR1 Antibody [NBP1-84590] - Analysis in human lymph node and pancreas tissues. Corresponding LAIR1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LAIR1-Antibody-Immunohistochemistry-Paraffin-NBP1-84590-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LAIR1 Antibody [NBP1-84590] - Analysis in human lymph node and pancreas tissues. Corresponding LAIR1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LAIR1 Antibody [NBP1-84590] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LAIR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84590-img0015.jpg)
![Immunohistochemistry-Paraffin: LAIR1 Antibody [NBP1-84590] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/LAIR1-Antibody-Immunohistochemistry-Paraffin-NBP1-84590-img0021.jpg)
![Immunohistochemistry-Paraffin: LAIR1 Antibody [NBP1-84590] - Staining of human colon shows moderate to strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/LAIR1-Antibody-Immunohistochemistry-Paraffin-NBP1-84590-img0018.jpg)
![Immunohistochemistry-Paraffin: LAIR1 Antibody [NBP1-84590] - Staining of human lung shows strong membranous positivity in macrophages.](http://images.novusbio.com/fullsize/LAIR1-Antibody-Immunohistochemistry-Paraffin-NBP1-84590-img0019.jpg)
![Immunohistochemistry-Paraffin: LAIR1 Antibody [NBP1-84590] - Staining of human lymph node shows strong membranous positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/LAIR1-Antibody-Immunohistochemistry-Paraffin-NBP1-84590-img0020.jpg)
![Western Blot: LAIR1 Antibody [NBP1-68845] - Analysis of LAIR1 in Human Lymph Node lysate (35ug protein in RIPA buffer) using NBP1-68845 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/LAIR1-Antibody-Western-Blot-NBP1-68845-img0001.jpg "Western Blot: LAIR1 Antibody [NBP1-68845] - Analysis of LAIR1 in Human Lymph Node lysate (35ug protein in RIPA buffer) using NBP1-68845 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: LAIR1 Antibody [NBP1-68845] - 3.75ug/ml staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/LAIR1-Antibody-Immunohistochemistry-Paraffin-NBP1-68845-img0002.jpg)
![Flow Cytometry: LAIR1 Antibody (113) [NBP1-43324] - Staining of C57BL/6 splenocytes with 0.125 ug of Armenian Hamster IgG Isotype Control Purified (purple line) or 0.125 ug of Anti-Mouse CD305 (LAIR-1) Purified (blue line) followed by Anti-Armenian Hamster IgG PE. Total viable F4/80+ cells were used for analysis.](http://images.novusbio.com/fullsize/LAIR1-Antibody-113-Flow-Cytometry-NBP1-43324-img0002.jpg "Flow Cytometry: LAIR1 Antibody (113) [NBP1-43324] - Staining of C57BL/6 splenocytes with 0.125 ug of Armenian Hamster IgG Isotype Control Purified (purple line) or 0.125 ug of Anti-Mouse CD305 (LAIR-1) Purified (blue line) followed by Anti-Armenian Hamster IgG PE. Total viable F4/80+ cells were used for analysis.")
![Immunohistochemistry: LAIR1 Antibody (PSH04-50) [NBP3-32517] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Mouse anti-LAIR1 antibody (NBP3-32517) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32517) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32517_mouse-lair1-mab-psh04-50-29820241492641.jpg "Immunohistochemistry: LAIR1 Antibody (PSH04-50) [NBP3-32517] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Mouse anti-LAIR1 antibody (NBP3-32517) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32517) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32517 at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: HL-60 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (negative) (20 µg/Lane) Predicted band size: 32 kDa Observed band size: 40-45 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32517 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32517_-western-blot-639204590071284097.jpg)
![Western Blot: LAIR1 Antibody [NBP1-52010] - (2ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/LAIR1-Antibody-Western-Blot-NBP1-52010-img0001.jpg "Western Blot: LAIR1 Antibody [NBP1-52010] - (2ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
