Human L1CAM ELISA Kit (Colorimetric) Summary
| Specificity |
Recognizes both recombinant and natural Human L1CAM. |
| Standard Curve Range |
39.06-2500 pg/ml (example only; lot dependent) |
| Sensitivity |
8.31 pg/ml |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 7 % |
| Intra-Assay |
CV% < 4.8 |
| Spike Recovery |
81-108% |
| Sample Volume |
100 ul |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
L1CAM |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage is content dependent. |
Kit Components
|
Components
|
- 1. 96 well microplate coated with Capture Antibody
- 2. Detection Antibody conjugated to HRP
- 3. Standards
- 4. Wash Buffer Concentrate
- 5. Dilution Buffer Concentrate
- 6. Color Reagent A
- 7. Color Reagent B
- 8. Stop Solution
|
Alternate Names for Human L1CAM ELISA Kit (Colorimetric)
Background
The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for L1CAM ELISA Kit (NBP2-80312). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.