Kynurenine 3-Monooxygenase/KMO Antibody - Azide and BSA Free

Western Blot: Kynurenine 3-Monooxygenase/KMO Antibody [NBP1-44263] - Lane 1: Brain Extract. Load: 10 ug per lane. Primary antibody: KMO antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 58 kDa for KMO. Other band(s): None.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Kynurenine 3-Monooxygenase/KMO Antibody - Azide and BSA Free Summary

• Description: This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Kynurenine 3-Monooxygenase/Kynurenine 3-Monooxygenase/KMO antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding 300-400aa of the mouse Kynurenine 3-Monooxygenase/KMO chain. (Uniprot: O15229)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KMO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:60000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Western Blot 1 ug/ml
• Application Notes: This product is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~58 kDa corresponding to the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with Mouse based on 100% sequence homology. Cross-reactivity with Kynurenine 3-Monooxygenase/KMO from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for Kynurenine 3-Monooxygenase/KMO Antibody - Azide and BSA Free

• KMO
• kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
• Kynurenine 3Monooxygenase
• Kynurenine 3-Monooxygenase

Background

Kynurenine 3-monooxygenase (KMO) catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). The enzyme is required for synthesis of quinolinic acid. Quinolinic acid is a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kynurenine 3-Monooxygenase/KMO Antibody (NBP1-44263)(2)

Publications using NBP1-44263

• Alexandre G. Maganin, Guilherme R. Souza, Miriam D. Fonseca, Alexandre H. Lopes, Rafaela M. Guimarães, André Dagostin, Nerry T. Cecilio, Atlante S. Mendes, William A. Gonçalves, Conceição E.A. Silva, Francisco Isaac Fernandes Gomes, Lucas M. Mauriz Marques, Rangel L. Silva, Letícia M. Arruda, Denis A. Santana, Henrique Lemos, Lei Huang, Marcela Davoli-Ferreira, Danielle Santana-Coelho, Morena B. Sant’Anna, Ricardo Kusuda, Jhimmy Talbot, Gabriela Pacholczyk, Gabriela A. Buqui, Norberto P. Lopes, Jose C. Alves-Filho, Ricardo M. Leão, Jason C. O’Connor, Fernando Q. Cunha, Andrew Mellor, Thiago M. Cunha Meningeal dendritic cells drive neuropathic pain through elevation of the kynurenine metabolic pathway in mice The Journal of Clinical Investigation 2022-12-01 [PMID: 36227694]
• Vamos E, Pardutz A, Klivenyi P et al. he role of kynurenines in disorders of the central nervous system: Possibilities for neuroprotection. J Neurol Sci 2009-01-01 [PMID: 19268309]

Bioinformatics

• Gene Symbol: KMO
• Entrez:
  • 8564(Human)
• Uniprot:
  • O15229()
  • O15229()
  • O15229()
  • O15229()
  • O15229()