Kynureninase Antibody

Western blot shows lysates of mouse liver tissue and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Mouse Kynureninase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7389) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Kynureninase at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Kynureninase Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Kynureninase
  Gly267-Ser464
  Accession # Q9CXF0
• Specificity: Detects mouse and rat Kynureninase in Western blots. Detects recombinant mouse Kynureninase in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human Kynureninase is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: KYNU
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kynureninase Antibody

• EC 3.7.1.3
• KYNU
• kynureninase (L-kynurenine hydrolase)
• Kynureninase
• L-kynurenine hydrolase

Background

Kynu (kyureninase (a hydrolase that acts on dog [Greek: kyon] urine [Greek: ouron]) is a 52-55 kDa member of the kyureninase family of enzymes. It is a pyridoxal phosphate-dependent cytosolic hydrolase that occurs in a variety of cell types, including hepatocytes and macrophages. Kynu participates in the metabolism of tryptophan. Dietary Trp is either incorporated into protein, or metabolized into niacin, serotonin or NAD. Kynu catalyzes one of two steps that lead to the formation of a key downstream intermediate called 3-hydroxyanthranilic acid. Mouse Kynu is 464 amino acids (aa) in length. It contains one large catalytic site (aa 24-462), and typically functions as a noncovalent homodimer. There are at least two potential isoform variants. One contains a three aa substitution for aa 425-464, while another possesses a 22 aa substitution for aa 302-464. Over aa267-464 mouse Kynu shares 91% and 80% aa sequence identity with rat and human Kynu, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KYNU
• Uniprot:
  • Q9CXF0()