Ku80/XRCC5 Antibody

Product Discontinued

Ku80/XRCC5 Antibody Summary

• Immunogen: Peptide with sequence C-SLAKKDEKTDTLED corresponding to internal region according to NP_066964.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: XRCC5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 80-85 kDa band observed in lysates of HeLa (calculated MW of 82.7 kDa band according to NP_066964.1). An additional band of 75 kDa band was observed consistent with products from other sources.
• Control:

  HeLa Whole Cell Lysate
  Ku80/XRCC5 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ku80/XRCC5 Antibody

• 80kDa
• ATP-dependent DNA helicase 2 subunit 2
• ATP-dependent DNA helicase II 80 kDa subunit
• CTC85
• CTCBF
• EC 3.6.4.-
• FLJ39089
• G22P2
• KARP1
• KARP-1
• Ku80
• Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen
• Ku86
• Ku86DNA repair protein XRCC5
• KUB2
• NFIV
• Thyroid-lupus autoantigen
• TLAA
• X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit
• X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD)
• X-ray repair cross-complementing protein 5
• X-ray repair, complementing defective, repair in Chinese hamster
• XRCC5

Background

Eukaryotic cells contain an abundant nuclear protein, Ku, which was initially discovered as an autoantigen. Ku is a heterodimer of 70 and 80 kDa subunits which binds to DNA ends and other alterations in double-stranded DNA including hairpins, nicks and gaps. Ku plays a role in DNA repair as an essential subunit of DNA-dependent protein kinase (DNA-PK) that phosphorylates certain transcription factors (Sp1, Oct-1 and p53). Ku targets DNA-PK to DNA. The Ku/DNA-PK complex might provide a structural framework for holding broken chromosomes together during repair. Ku plays a role in many diverse processes including cell signaling, proliferation, DNA repair, replication, transcriptional activation and apoptosis (2-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ku80/XRCC5 Antibody (NB100-2840)(1)

Publication using NB100-2840

• Mayeur GL, Kung WJ, Martinez A et al. Ku is a novel transcriptional recycling coactivator of the androgen receptor in prostate cancer cells. J Biol Chem 2005-03-18 [PMID: 15640154]

Bioinformatics

• Gene Symbol: XRCC5
• Entrez:
  • 7520(Human)
• Uniprot:
  • NP_066964.1(Human)