Ku80/XRCC5 Antibody [Alexa Fluor® 532]

• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 532

Ku80/XRCC5 Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human Ku80
  Asn402-Lys565
  Accession # P13010
• Specificity: Detects human Ku80 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Ku80 (Lupus Ku autoantigen protein p86; also ATP-dependent DNA helicase 2 subunit 2 and Ku86) is a ubiquitous 80 - 85 kDa nuclear protein that serves as a component of DNA-dependent protein kinase. In particular, it heterodimerizes with Ku70 to form a complex that binds DNA ends and select sequences, and which recruits DNA-PKcs for the purpose of phosphorylating multiple chromatin-bound proteins, including p53, Oct-1 and Sp-1. The Ku:DNA-PKcs complex participates in DNA repair, DNA replication, and VDJ recombination. Human Ku80 is 732 amino acids (aa) in length. It contains a Ku domain (aa 244 - 543) with a DNA binding motif (aa 257 - 268), and a C-terminal DNA-PKcs-binding sequence (aa 720 - 728). Over aa 402 - 565, Ku80 shares 77% aa identity with mouse Ku80.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.