Ku80/XRCC5 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Ku80/XRCC5 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Ku80
  Asn402-Lys565
  Accession # P13010
• Specificity: Detects human Ku80 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ku80/XRCC5 Antibody [Alexa Fluor® 488]

• 80kDa
• ATP-dependent DNA helicase 2 subunit 2
• ATP-dependent DNA helicase II 80 kDa subunit
• CTC85
• CTCBF
• EC 3.6.4.-
• FLJ39089
• G22P2
• KARP1
• KARP-1
• Ku80
• Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen
• Ku86
• Ku86DNA repair protein XRCC5
• KUB2
• NFIV
• Thyroid-lupus autoantigen
• TLAA
• X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit
• X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD)
• X-ray repair cross-complementing protein 5
• X-ray repair, complementing defective, repair in Chinese hamster
• XRCC5

Background

Ku80 (Lupus Ku autoantigen protein p86; also ATP-dependent DNA helicase 2 subunit 2 and Ku86) is a ubiquitous 80 - 85 kDa nuclear protein that serves as a component of DNA-dependent protein kinase. In particular, it heterodimerizes with Ku70 to form a complex that binds DNA ends and select sequences, and which recruits DNA-PKcs for the purpose of phosphorylating multiple chromatin-bound proteins, including p53, Oct-1 and Sp-1. The Ku:DNA-PKcs complex participates in DNA repair, DNA replication, and VDJ recombination. Human Ku80 is 732 amino acids (aa) in length. It contains a Ku domain (aa 244 - 543) with a DNA binding motif (aa 257 - 268), and a C-terminal DNA-PKcs-binding sequence (aa 720 - 728). Over aa 402 - 565, Ku80 shares 77% aa identity with mouse Ku80.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.