KRT9 Antibody (SD201-09)

Western Blot: KRT9 Antibody (SD201-09) [NBP2-67810] - Analysis of KRT9 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: KRT9 Antibody (SD201-09) [NBP2-67810] - Analysis of MCF-7 cells labeling KRT9 (and then blocked with 2% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody KRT9 (red) at 1/200 dilution for overnight at 4 . Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibody at 1/800 dilution. DAPI was used to stain the cell nuclei (blue).

Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling KRT9 (washed with PBS. And then probed with the primary antibodies KRT9 (red) at 1/50 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810] - Analysis of mouse skin tissue using anti-Cytokeratin 9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: KRT9 Antibody (SD201-09) [NBP2-67810] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling KRT9 washed with PBS. And then probed with the primary antibodies KRT9 (red) at 1/50 dilution and Vimentin (green) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: SD201-09
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

KRT9 Antibody (SD201-09) Summary

• Description: Novus Biologicals Rabbit KRT9 Antibody (SD201-09) (NBP2-67810) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein wiehin Human KRT9 aa 234-422 / 623. (SwissProt: P35527 Human; SwissProt: Q6RHW0 Mouse; SwissProt: Q8CIS9 Rat)
• Localization: Extracellular exosome, membrane, nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for KRT9 Antibody (SD201-09)

• cytokeratin-9
• EPPK
• keratin 9
• keratin, type I cytoskeletal 9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT9