Kremen-1 Antibody [Unconjugated]

Kremen-1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Kremen-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2127) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Kremen-1 was detected in immersion fixed paraffin-embedded sections of human colon array using Goat Anti-Human Kremen-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2127) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Kremen-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Kremen‑1
  Ala20-Gly397
  Accession # NP_114434
• Specificity: Detects human Kremen-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Kremen-1 and less than 5% cross-reactivity with recombinant human Kremen-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KREMEN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kremen-1 Antibody [Unconjugated]

• EC 3.4.21
• FLJ31863
• KREMEM1
• kremen protein 1
• KREMEN
• Kremen1
• Kremen-1
• kringle containing transmembrane protein 1
• kringle containing transmembrane protein
• Kringle domain-containing transmembrane protein 1
• kringle-coding gene marking the eye and the nose
• Kringle-containing protein marking the eye and the nose
• KRM1
• KRM1Dickkopf receptor

Background

Kremen-1 is a type I transmembrane protein that contains extracellular Kringle, WSC, and CUB domains. It is expressed in diverse embryonic and adult tissues. Kremen proteins bind the secreted Dickkopf proteins Dkk-1, -2, and -4 with high affinity to antagonize the canonical Wnt signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kremen-1 Antibody (AF2127)(2)

Publications using AF2127

• Cooney, RA;Saal, ML;Geraci, KP;Maynard, C;Cleaver, O;Hoang, ON;Moore, TT;Hwang, RF;Axelrod, JD;Vladar, EK; A WNT4 and DKK3 driven canonical to noncanonical Wnt signaling switch controls multiciliogenesis Journal of cell science 2023-07-28 [PMID: 37505110] (Immunocytochemistry, Human)
• E.-M. Pfaff, S. Becker, A. Gunther, M. Konigshoff Dickkopf proteins influence lung epithelial cell proliferation in idiopathic pulmonary fibrosis European Respiratory Journal 1/1/2011 [PMID: 20650998]

Bioinformatics

• Gene Symbol: KREMEN1
• Uniprot:
  • NP_114434()