KMT3B/NSD1 Antibody

Immunohistochemistry-Paraffin: KMT3B/NSD1 Antibody [NB100-1744] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NSD1 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit .

Immunohistochemistry-Paraffin: KMT3B/NSD1 Antibody [NB100-1744] - Sample : FFPE section of human prostate adenocarcinoma. Antibody: Affinity purified rabbit anti-NSD1 used at a dilution of 1:250. Detection: DAB

• Reactivity: Hu
• Applications: IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.25 mg/ml

KMT3B/NSD1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 650 and 700 of human NSD1 using the numbering given in SwissProt entry Q96L73 (GeneID 64324).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NSD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
• Application Notes: Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.25 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KMT3B/NSD1 Antibody

• Androgen receptor coactivator 267 kDa protein
• Androgen receptor-associated protein of 267 kDa
• ARA267STO
• DKFZp666C163
• EC 2.1.1.43
• FLJ10684
• FLJ22263
• FLJ44628
• H3 lysine-36 and H4 lysine-20 specific
• KMT3BSotos syndrome
• Lysine N-methyltransferase 3B
• NR-binding SET domain-containing protein
• nuclear receptor binding SET domain protein 1

Background

KMT3B/NSD1 is a histone methyltransferase that preferentially methylates Lys-36 of histone H3 and Lys-20 of histone H4. KMT3B/NSD1 can function as an androgen receptor coactivator and is capable of both negatively and positively influencing transcription. Mutations in KMT3B/NSD1 are responsible for Sotos syndrome, Weaver syndrome, Beckwith-Wiedemann syndrome, and a form of childhood leukemia. Alternate names for KMT3B/NSD1 include nuclear receptor binding SET domain protein 1, ARA267, ARA-267 androgen receptor-associated coregulator 267, STO, SOTOS, Sotos syndrome, and FLJ22263.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KMT3B/NSD1 Antibody (NB100-1744)(1)

Publication using NB100-1744

• Quintana RM, Dupuy AJ, Bravo A et al. A transposon-based analysis of gene mutations related to skin cancer development. J Invest Dermatol 2013-01-01 [PMID: 22832494]

Bioinformatics

• Gene Symbol: NSD1
• Entrez:
  • 64324(Human)
• Uniprot:
  • Q96L73(Human)