Klre-1 Antibody [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

Klre-1 Antibody [Alexa Fluor® 594] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Klre-1
  Lys94-Lys226
  Accession # NP_705818
• Specificity: Detects mouse Klre-1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human NKG2D and recombinant mouse NKG2D is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Klre-1 Antibody [Alexa Fluor® 594]

• CD94
• Klre1
• Klre-1
• NKG2I

Background

NKG2I (NKG2 family member I; also Klre-1) is a 24-28 kDa member of the NKG2/KLR family of proteins. It is expressed on mouse NK and NKT cells, and appears to serve as one component of two novel heterodimeric cell surface receptors. When complexed to KLRI1, NKG2I inhibits NK cell cytotoxic activity. When complexed to KLRI2, NKG2I activates NK cells, inducing IFN-gamma production and the activation of a cytolytic program. Although NKG2I preferentially associates noncovalently with NLRI1 and I2, it apparently will form disulfide-linked homodimers in the absence of its heterodimer partners. Mouse NKG2I is a 226 amino acid (aa) type II transmembrane protein. It contains an N-terminal cytoplasmic segment (aa 1-68) plus a 133 aa extracellular region (aa 94-226) that possesses one C-type lectin domain (aa 110-226). Over aa 94-226, mouse NKG2I shares 82% aa identity with rat NKG2I. There does not appear to be a human structural ortholog to mouse NKG2I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.