Klotho Antibody (JM93-76)

Western Blot: Klotho Antibody (JM93-76) [NBP2-75551] - Analysis of Klotho on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human serum lysateLane 2: human kidney tissue lysate

Immunohistochemistry-Paraffin: Klotho Antibody (JM93-76) [NBP2-75551] - Analysis of paraffin-embedded mouse kidney tissue using anti-Klotho antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Klotho Antibody (JM93-76) [NBP2-75551] - Analysis of paraffin-embedded human kidney tissue using anti-Klotho antibody. Counter stained with hematoxylin.

Product Discontinued

Klotho Antibody (JM93-76) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Klotho aa 404-453 / 1,012. (SwissProt: Q9UEF7 Human; SwissProt: O35082 Mouse)
• Localization: Cell membrane. Apical cell membrane. Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Klotho Antibody (JM93-76)

• EC 3.2.1
• EC 3.2.1.31
• KL
• Klotho

Background

The Klotho gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Klotho Antibody (NBP2-75551). (Showing 1 - 1 of 1 FAQ).

1. I study physiology (PhD) and I need a klotho kit.
   • A list of our klotho products can be found here. I am not entirely sure what you are looking for as I don't have your complete message for some reason. Can you please clarify what you are looking for?

Bioinformatics

• Gene Symbol: KL