KLHL2 Antibody

Western Blot: KLHL2 Antibody [NBP2-17080] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE gel, diluted at 1:5000.

Immunocytochemistry/Immunofluorescence: KLHL2 Antibody [NBP2-17080] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using KLHL2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: KLHL2 Antibody [NBP2-17080] - Human colon carcinoma, using KLHL2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

KLHL2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human KLHL2. The exact sequence is proprietary.
• Localization: Cytoplasm; cytoskeleton
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KLHL2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for KLHL2 Antibody

• ABP-KELCH
• Actin-binding protein Mayven
• kelch (Drosophila)-like 2 (Mayven)
• kelch
• kelch-like 2, Mayven (Drosophila)
• kelch-like protein 2
• MAV
• MAYVEN

Background

KLHL2, also known as Kelch-like protein 2, has a 593 short amino acid that is 66 kDa and a long 597 amino acid that is 67 kDa, predominantly expressed in brain, and may participate in organizing the actin cytoskeleton of the brain cells. This protein is currently being studied for research on the following diseases and disorders: contagious pustular dermatitis, lumpy skin disease, monkeypox, smallpox, cowpox, vaccinia, skin disease, dermatitis, pharyngitis, breast cancer, and neuronitis. The protein has been shown to interact with KLHL12 in intracellular protein transport pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for KLHL2 Antibody (NBP2-17080)(1)

Publication using NBP2-17080

• Zhang E, Dong X, Chen S et al. Ubiquitin ligase KLHL2 promotes the degradation and ubiquitination of ARHGEF7 protein to suppress renal cell carcinoma progression Am J Cancer Res 2020-10-01 [PMID: 33163274] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: KLHL2
• Uniprot:
  • O95198(Human)