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KLHL2 Antibody

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Western Blot: KLHL2 Antibody [NBP2-17080] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE gel, diluted at 1:5000.
Western Blot: KLHL2 Antibody [NBP2-17080] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: KLHL2 Antibody [NBP2-17080] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using KLHL2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled ...read more
Immunohistochemistry-Paraffin: KLHL2 Antibody [NBP2-17080] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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KLHL2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human KLHL2. The exact sequence is proprietary.
Localization
Cytoplasm; cytoskeleton
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KLHL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for KLHL2 Antibody

  • ABP-KELCH
  • Actin-binding protein Mayven
  • kelch (Drosophila)-like 2 (Mayven)
  • kelch
  • kelch-like 2, Mayven (Drosophila)
  • kelch-like protein 2
  • MAV
  • MAYVEN

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Nrf2 Antibody [NBP1-32822] - Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody diluted by 1:1000.
Immunocytochemistry/Immunofluorescence: Nrf2 Antibody [NBP1-32822] - NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: Nrf2 Antibody [NBP1-32822] - Paraffin-embedded Cal27 xenograft, using Nrf2 antibody at 1:500 dilution.
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Western Blot: Keap1 Antibody (1B4) [NBP2-03319] - Analysis of human BEAS2B cells.
Immunohistochemistry-Paraffin: KEAP1 Antibody (1B4) [NBP2-03319] - Staining of paraffin-embedded Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody.
Flow Cytometry: KEAP1 Antibody (1B4) [NBP2-03319] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KEAP1 antibody, and then analyzed by flow cytometry.
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Western Blot: Cullin 3 Antibody [NB100-58788] - Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit anti-Cul3 antibodies(recognize downstream epitopes).
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Western Blot: GABPA Antibody (M1) [H00002551-M03] - Using 15 ug of human glioblastoma stem cells lysates. Primary antibody: GABPA dilution at 1:500 in 2.5% BSA/TBST. This image was submitted via customer Review.
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Western Blot: Exosome component 10 Antibody [NBP1-32870] - analysis of EXOSC10 in HeLa whole cell extracts treated with (1) si-control and (2) EXOSC10 siRNA using anti-EXOSC10 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.
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Western Blot: ALDH9A1 Antibody [NBP1-89150] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: ALDH9A1 Antibody [NBP1-89150] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Western Blot: ALDH9A1 Antibody [NBP1-89150] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
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Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:1000.
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Western Blot: KLHL1 Antibody [NBP1-76670] - Analysis of KLHL1 in HeLa cell lysate with KLHL1 antibody at (A) 2 and (B) 4 ug/mL.
Immunohistochemistry-Paraffin: KLHL1 Antibody [NBP1-76670] - Rat brain tissue with KLHL1 antibody at 10 ug/ml.
Western Blot: KLHL1 Antibody [NBP1-76670] - Analysis of KLHL1 in Human brain cell lysate with KLHL1 antibody using NBP1-76670 at (A) 2 and (B) 4 ug/ml.
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Western Blot: NS1-BP Antibody [NBP2-24567] - Analysis of Influenza A NS in a partial recombinant fusion protein containing amino acids DQKLGDAPFLDRLRRD using this antibody (1 ug/ml). Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
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KLHL2 Antibody
NBP2-17080
KLHL2 Antibody
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

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Product General Protocols

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Bioinformatics

Gene Symbol KLHL2
Uniprot