KLHL12/C3IP1 Antibody (OTI4E1) [DyLight 650]

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: OTI4E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 650

KLHL12/C3IP1 Antibody (OTI4E1) [DyLight 650] Summary

• Immunogen: Recombinant protein fragment corresponding to amino acids 177-405 of human KLHL12/C3IP1 (NP_067646) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLHL12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for KLHL12/C3IP1 Antibody (OTI4E1) [DyLight 650]

• C3IP1kelch-like protein 12
• CUL3-interacting protein 1
• DKIR
• FLJ27152
• kelch-like 12 (Drosophila)
• kelch-like protein C3IP1

Background

Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3. Component of an ubiquitin-protein E3 ligase complex which includes at least CUL3 and KLHL12. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLHL12