KLHL12/C3IP1 Antibody (OTI4E1) - Azide and BSA Free

Western Blot: KLHL12/C3IP1 Antibody (OTI4E1) - Azide and BSA Free [NBP2-72118] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL12.

Immunohistochemistry: KLHL12/C3IP1 Antibody (OTI4E1) - Azide and BSA Free [NBP2-72118] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: KLHL12/C3IP1 Antibody (OTI4E1) - Azide and BSA Free [NBP2-72118] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: KLHL12/C3IP1 Antibody (OTI4E1) - Azide and BSA Free [NBP2-72118] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: KLHL12/C3IP1 Antibody (OTI4E1) - Azide and BSA Free [NBP2-72118] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI4E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

KLHL12/C3IP1 Antibody (OTI4E1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse KLHL12/C3IP1 Antibody (OTI4E1) - Azide and BSA Free (NBP2-45512) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 177-405 of human KLHL12 (NP_067646) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLHL12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Theoretical MW: 63.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for KLHL12/C3IP1 Antibody (OTI4E1) - Azide and BSA Free

• C3IP1kelch-like protein 12
• CUL3-interacting protein 1
• DKIR
• FLJ27152
• kelch-like 12 (Drosophila)
• kelch-like protein C3IP1

Background

Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3. Component of an ubiquitin-protein E3 ligase complex which includes at least CUL3 and KLHL12. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLHL12