KLF5 Antibody (HL2269) - Azide and BSA Free

Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - Wild-type (WT) and KLF5 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - Whole cell extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - KLF5 antibody [HL2269] detects KLF5 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KLF5 stained by KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - KLF5 antibody [HL2269] detects KLF5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. KLF5 stained by KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: HL2269
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

KLF5 Antibody (HL2269) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit KLF5 Antibody (HL2269) - Azide and BSA Free (NBP3-25544) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human KLF5.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KLF5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for KLF5 Antibody (HL2269) - Azide and BSA Free

• Basic transcription element-binding protein 2
• BTEB2
• BTE-binding protein 2
• CKLFGC-box-binding protein 2
• Colon krueppel-like factor
• colon kruppel-like factor
• GC box binding protein 2
• IKLFbasic transcription element binding protein 2
• Intestinal-enriched krueppel-like factor
• intestinal-enriched kruppel-like factor
• KLF5
• Krueppel-like factor 5
• Kruppel-like factor 5 (intestinal)
• Transcription factor BTEB2

Background

KLF5 encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLF5