Kir6.2 Antibody

Western Blot: Kir6.2 Antibody [NBP1-00206] - (0.1ug/ml) staining of Human Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Kir6.2 Antibody [NBP1-00206] - (3.8ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Kir6.2 Antibody Summary

• Immunogen: Peptide with sequence C-AEDPAKPRYRARQ corresponding to internal region (near N-Terminus) according to NP_000516.3.
• Specificity: This antibody is expected to recognise isoform 1 (NP_000516.3) only.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KCNJ11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 45-48 kDa band observed in human skeletal muscle lysates (calculated MW of 43.5 kDa band according to NP_000516.3). IHC-P: Human pancreas shows variable staining across the islet of langerhans.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kir6.2 Antibody

• ATP-sensitive inward rectifier potassium channel 11
• beta-cell inward rectifier subunit
• BIRKIR6.2
• HHF2
• IKATP
• Inward rectifier K(+) channel Kir6.2
• inwardly rectifying potassium channel KIR6.2
• KCNJ11
• Kir6.2
• MGC133230
• PHHI
• potassium channel inwardly rectifing subfamily J member 11
• Potassium channel, inwardly rectifying subfamily J member 11
• potassium inwardly-rectifying channel, subfamily J, member 11
• TNDM3

Background

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and is found associated with the sulfonylurea receptor SUR. Mutations in this gene are a cause of familial persistent hyperinsulinemic hypoglycemia of infancy (PHHI), an autosomal recessive disorder characterized by unregulated insulin secretion. Defects in this gene may also contribute to autosomal dominant non-insulin-dependent diabetes mellitus type II (NIDDM), transient neonatal diabetes mellitus type 3 (TNDM3), and permanent neonatal diabetes mellitus (PNDM). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kir6.2 Antibody (NBP1-00206)(1)

Publication using NBP1-00206

• Filosa JA, Bonev AD, Straub SV et al. Local potassium signaling couples neuronal activity to vasodilation in the brain. Nat Neurosci 2006-11-01 [PMID: 17013381]

Bioinformatics

• Gene Symbol: KCNJ11
• Entrez:
  • 3767(Human)
• Uniprot:
  • NP_000516.3(Human)