Kir3.4 Antibody

Western Blot: Kir3.4 Antibody [NBP2-76943] - Western blot analysis of Kir3.4 on different lysates using anti-Kir3.4 antibody at 1/500 dilution.Positive control:Lane1: Mouse heart Lane2: Rat heart

Immunocytochemistry/Immunofluorescence: Kir3.4 Antibody [NBP2-76943] - ICC staining Kir3.4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Kir3.4 Antibody [NBP2-76943] - Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin.

Flow Cytometry: Kir3.4 Antibody [NBP2-76943] - Flow cytometric analysis of HepG2 cells with Kir3.4 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Kir3.4 Antibody [NBP2-76943] - ICC staining Kir3.4 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Kir3.4 Antibody [NBP2-76943] - ICC staining Kir3.4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Kir3.4 Antibody [NBP2-76943] - Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin.

Immunohistochemistry: Kir3.4 Antibody [NBP2-76943] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin.

Immunohistochemistry: Kir3.4 Antibody [NBP2-76943] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin.

Product Discontinued

Kir3.4 Antibody Summary

• Immunogen: Synthetic peptide within N-terminal human Kir3.4. (SwissProt: P48544 Human; SwissProt: P48545 Mouse; SwissProt: P48548 Rat)
• Localization: Membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCNJ5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kir3.4 Antibody

• Cardiac inward rectifier
• CIRKIR3.4
• G protein-activated inward rectifier potassium channel 4
• GIRK-4
• GIRK4Kir3.4
• Heart KATP channel
• Inward rectifier K(+) channel Kir3.4
• inward rectifier K+ channel KIR3.4
• IRK-4
• KATP-1
• KATP1cardiac ATP-sensitive potassium channel
• LQT13
• Potassium channel, inwardly rectifying subfamily J member 5
• potassium inwardly-rectifying channel, subfamily J, member 5

Background

G-protein regulated inward-rectifier potassium channels (GIRK) are part of a superfamily of inward-rectifier K+ channels. To date four GIRK subunits, designated GIRK1-4 (also designated Kir3.1-4), have been identified in mammals, and GIRK5 has been found in Xenopus oocytes. GIRK channels exist in vivo both as homotetramers and heterotetramers. GIRK channels are modulated by G-proteins; they are also modulated by phosphatidylinositol 4,5-bisphosphate, intracellular sodium, ethanol and mechanical stretch. GIRK1, 2 and 3 are highly abundant in brain. In general, neuronal GIRK channels are involved in the regulation of the excitability of neurons and may contribute to the resting potential.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KCNJ5