KIR2DS1/CD158h Antibody (1127B) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) were stained with Rabbit Anti-Human KIR2DS1/CD158h PE-conjugated Monoclonal Antibody (Catalog # FAB8887P) and either (A) Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) or (B) Mouse Anti-Human KIR2DL1/KIR2DS5 APC-conjugated Monoclonal Antibody (Catalog # FAB1844A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 1127B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Phycoerythrin

KIR2DS1/CD158h Antibody (1127B) [Phycoerythrin] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: HEK293 human embryonic kidney cell line transfected with human KIR2DS1/CD158h
  Met1-His245
  Accession # Q14954
• Specificity: Detects human KIR2DS1/CD158h in flow cytometry. Clone 1127B recognizes KIR2DS1 and some alleles of KIR2DL1, both members of the killer cell immunoglobulin-like receptor (KIR) family. Because Clone 1127B displays partial cross-reactivity with KIR2DL1, co-staining with Mouse Anti-Human KIR2DL1 Clone 143211 (Catalog # FAB1844A) is recommended.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KIR2DS1
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KIR2DS1/CD158h Antibody (1127B) [Phycoerythrin]

• killer cell immunoglobulin-like receptor 2DS1
• CD158a
• CD158h
• EB6ActI
• killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 1
• KIR2DS1
• p50.1

Background

The KIRs comprise a family of 14 polymorphic and homologous activating and inhibitory receptors expressed primarily on CD56^dim NK cells. KIR2DS1 is an activating receptor with high homology to the inhibitory receptor KIR2DL1. Both KIR2DL1 and KIR2DS1 bind to HLA-C alleles containing the C2 epitope. KIR2DS1 and KIR2DL1 expression regulates NK cell licensing and activation in a number of immune contexts. Over amino acids (aa) 1-245, human KIR2DS1 shares 97% aa sequence identity with human KIR2DL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KIR2DS1/CD158h Antibody (FAB8887P)(1)

Publication using FAB8887P

• O Huhn, O Chazara, MA Ivarsson, C Retière, TC Venkatesan, PJ Norman, HG Hilton, J Jayaraman, JA Traherne, J Trowsdale, M Ito, C Kling, P Parham, H Ghadially, A Moffett, AM Sharkey, F Colucci High-Resolution Genetic and Phenotypic Analysis of KIR2DL1 Alleles and Their Association with Pre-Eclampsia J. Immunol., 2018-09-24;0(0):. 2018-09-24 [PMID: 30249807] (Human)

Bioinformatics

• Gene Symbol: KIR2DS1
• Uniprot:
  • Q14954()